Magnetic reagent, magnetic reagent kit, method for treating magnetic carriers, and treatment device therefor

Patent metadata

Field	Value
Publication number	US-9694368-B2
Application number	US-201013509232-A
Country	US
Kind code	B2
Filing date	Nov 12, 2010
Priority date	Nov 13, 2009
Publication date	Jul 4, 2017
Grant date	Jul 4, 2017

Abstract

The present invention relates to a magnetic reagent, a magnetic reagent kit, a method for treating magnetic carriers, and a treatment device therefor, with an object of quickly and efficiently treating magnetic carriers of a micro particle diameter including nanosized magnetic carriers.
The magnetic reagent or magnetic reagent kit includes: a plurality of magnetic carriers which can be magnetized by being exposed to a magnetic field, can be bonded to a predetermined chemical substance or living organism in a liquid, and that have a particle diameter that enables them to be suspended in the liquid; and a plurality of treatment promoting magnetic particles which can be magnetized by being exposed to a magnetic field and thus can have the magnetic carriers adsorbed on the surface thereof, are formed so that they can be moved within the liquid by movement of the liquid or by a magnetic field, and promote a treatment for capturing or re-suspension of the magnetic carriers.

First claim

The invention claimed is:

1. A magnetic reagent kit having:
a plurality of magnetic carriers that are magnetizable by exposure to a magnetic field, and bonded, in a liquid, to either a predetermined chemical substance or a living organism, said magnetic carriers each having a particle diameter that enables it to be suspended in the liquid;
a plurality of treatment promoting magnetic particles that are magnetizable by exposure to the magnetic field so that said magnetic carriers are adsorbable on respective surfaces thereof, said treatment promoting magnetic particles being formed so as to be suspended, or dispersed, within said liquid by the magnetic field, or by movement of said liquid, to promote a treatment for capturing or re-suspension of said magnetic carriers; and
a cartridge container having one or more liquid accommodating parts and one or more tip accommodating parts, the one or more liquid accommodating parts defining an opening part sealed with a perforatable thin film, the one or more tip accommodating parts containing a dispensing tip and a perforation tip by which the perforatable thin film is perforatable, and the dispensing tip having a capturing region on which the magnetic carriers and the treatment promoting magnetic particles are adsorbable by exposure to the magnetic field,
wherein the treatment promoting magnetic particles have a magnetic flux density higher than that of the magnetic carriers, and are provided so as not to be bonded, in the liquid, to either the predetermined chemical substance or the living organism,
wherein the magnetic carriers are accommodated within at least one of the one or more liquid accommodating parts, and the treatment promoting magnetic particles are accommodated within the at least one of the one or more liquid accommodating parts, or at least another one of the one or more liquid accommodating parts,
wherein the plurality of treatment promoting magnetic particles, the plurality of magnetic carriers, and either the predetermined chemical substance or the living organism, are mixable in the liquid to form a mixture, said mixture being suctioned and discharged by the dispensing tip, and
wherein, during the suctioning and discharging of the mixture by the dispensing tip, the mixture is exposed to the magnetic field so that the treatment promoting magnetic particles and the magnetic carriers are adsorbed on the capturing region of the dispensing tip.

2. The magnetic reagent kit according to claim 1 , wherein a particle diameter of said treatment promoting magnetic particles is greater than that of said magnetic carriers.

3. The magnetic reagent kit according to claim 1 , wherein a particle diameter of said treatment promoting magnetic particles is in a range of 0.001 mm to 5 mm.

4. The magnetic reagent kit according to claim 3 , wherein a magnetic susceptibility of said treatment promoting magnetic particles is greater than that of said magnetic carriers at normal temperature.

5. The magnetic reagent kit according to claim 1 , wherein said treatment promoting magnetic particles have a ferromagnetic body, and said magnetic carriers have a paramagnetic body or a super paramagnetic body.

6. The magnetic reagent kit according to claim 1 , wherein said treatment promoting magnetic particles are formed with a size or from a material different from that of said magnetic carriers.

7. The magnetic reagent kit according to claim 2 , wherein a magnetic susceptibility of said treatment promoting magnetic particles is greater than that of said magnetic carriers at normal temperature.

8. The magnetic reagent kit according to claim 1 , wherein the treatment promoting magnetic particles adsorbed on the capturing region expand an effective reaching range of the magnetic field.

Assignees

• Tajima Hideji
• Universal Bio Research Co Ltd

Inventors

• Tajima Hideji

Classifications

• G01N33/54326

  Magnetic particles · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• B03C1/015 Primary

  by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation · CPC title