Integrated electrospray ionization emitter and detection cell for parallel measurements by fluorescence and mass spectrometry

Patent metadata

Field	Value
Publication number	US-9620348-B2
Application number	US-201313927650-A
Country	US
Kind code	B2
Filing date	Jun 26, 2013
Priority date	Mar 1, 2011
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

An integrated mass spectrometer electrospray emitter and fluorescence detector allows improved volumetric measurements of separate components from a liquid chromatography column by improving correlation between the readings of these instruments and reducing dead volume and sample size requirements.

First claim

We claim:

1. An electrospray emitter for mass spectrometry and fluorescent spectroscopy comprising:
a conduit having a substantially continuous portion extending along a conduit axis and terminating at an orifice adapted to provide an electrospray ionization for a mass spectrometer;
chromatographic packing material positioned within a chromatography portion of the continuous portion;
a first filter fitting within the conduit and sized to block the chromatographic packing material;
a second filter fitting within the conduit downstream from the first filter and sized to preserve a liquid pressure within the conduit; and
a transparent analysis region positioned within the continuous portion between the first filter and the second filter adapted for fluorescent spectroscopy measurement;
wherein a volume of a conduit between the orifice and the chromatography portion is less than 1 microliter.

2. The electrospray emitter assembly of claim 1 wherein the conduit is a glass capillary tube.

3. The electrospray emitter assembly of claim 1 wherein an internal diameter of the conduit is less than 1000 μm.

4. The electrospray emitter assembly of claim 1 wherein the first filter is a cast chemical frit.

5. An electrospray emitter for mass spectrometry and fluorescent spectroscopy comprising:
a conduit having a substantially continuous portion extending along a conduit axis and terminating at an orifice adapted to provide an electrospray ionization for a mass spectrometer;
chromatographic packing material positioned within a chromatography portion of the continuous portion;
a filter fitting within the conduit and sized to block the chromatographic packing material;
a bubble reducing element positioned in the conduit downstream from the filter; and
a transparent analysis region positioned within the continuous portion between the first fitting and the bubble reducing element adapted for fluorescent spectroscopy measurement;
wherein a volume of a conduit between the orifice and the chromatography portion is less than 1 microliter.

6. The electrospray emitter assembly of claim 5 wherein the conduit is a glass capillary tube.

7. The electrospray emitter assembly of claim 5 wherein an internal diameter of the conduit is less than 1000 μm.

8. The electrospray emitter assembly of claim 5 wherein the first filter is a cast chemical frit.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Russell Jason Dale
• Coon Joshua Jacques
• Hilger Ryan Tyler
• Smith Lloyd Michael
• Ladror Daniel T
• Shortreed Michael Robert
• Scalf Mark Andrew

Classifications

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• H01J49/167 Primary

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title

• G01N30/7266

  by electric field, e.g. electrospray · CPC title