Vaccines and diagnostics for the ehrlichioses

Patent metadata

Field	Value
Publication number	US-9605036-B2
Application number	US-201514724136-A
Country	US
Kind code	B2
Filing date	May 28, 2015
Priority date	Jan 10, 2008
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

The present invention concerns VLPT immunoreactive compositions for E. chaffeensis and compositions related thereto, including vaccines, antibodies, polypeptides, peptides, and polynucleotides. In particular, epitopes for E. chaffeensis VLPT are disclosed.

First claim

What is claimed is:

1. A method of detecting an anti- E. chaffeensis antibody in an individual, comprising the step of assaying a biological sample from the individual for the presence of an antibody that immunologically binds to a polypeptide comprising the amino acid sequence of SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; or SEQ ID NO: 11, or an amino acid sequence that is at least 95% identical to SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; or SEQ ID NO: 11, wherein the polypeptide is no more than 50 amino acids in length and the assaying comprises contacting the sample with the polypeptide.

2. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 3, or the amino acid sequence that is at least 95% identical thereto.

3. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 4, or the amino acid sequence that is at least 95% identical thereto.

4. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 5, or the amino acid sequence that is at least 95% identical thereto.

5. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 8, or the amino acid sequence that is at least 95% identical thereto.

6. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 9, or the amino acid sequence that is at least 95% identical thereto.

7. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 10, or the amino acid sequence that is at least 95% identical thereto.

8. The method of claim 1 , wherein the antibody immunologically binds to the amino acid sequence of SEQ ID NO: 11, or the amino acid sequence that is at least 95% identical thereto.

9. The method of claim 1 , wherein the polypeptide is immobilized on a solid support.

10. The method of claim 9 , wherein the solid support is a column matrix, a well of a microtiter plate, a dot blot membrane, or a Western blot membrane.

11. A method of detecting an anti- E. chaffeensis antibody in an individual comprising the step of assaying a biological sample from the individual for the presence of an antibody that immunologically binds to an isolated polypeptide consisting of one or more of the amino acid sequences selected from SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; or SEQ ID NO: 11, or an amino acid sequence that is at least 95% identical to SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; or SEQ ID NO: 11, wherein the assaying comprises contacting the sample with the polypeptide.

12. The method of claim 11 , wherein the method comprises:
(a) contacting the polypeptide with the biological sample under conditions effective to allow the formation of a polypeptide-antibody complex and
(b) detecting the polypeptide-antibody complex.

13. The method of claim 11 , wherein the method is a microtiter plate assay, enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA), immunoradiometric assay, fluoroimmunoassay, chemiluminescent assay, bioluminescent assay, paramagnetic assay, or a Western blot.

14. The method of claim 12 , wherein the method further comprises determining the amount of the antibody that immunologically binds to the polypeptide.

15. The method of claim 12 , wherein the polypeptide is immobilized on a solid support.

16. The method of claim 15 , wherein the solid support is a column matrix, a well of a microtiter plate, a dot blot membrane, or a Western blot membrane.

17. The method of claim 12 , wherein the individual is a mammal.

18. The method of claim 12 , wherein the individual is a canine.

Assignees

• Res Dev Foundation

Inventors

• Mcbride Jere W
• Luo Tian

Classifications

• C07K14/29 Primary

  from Richettsiales (O) · CPC title

• G01N2333/195

  from bacteria · CPC title

• G01N2333/29

  from Richettsiales (o) · CPC title

• C07K16/1246 Primary

  Rickettsiales (O) · CPC title

• A61K39/0233

  Rickettsiales, e.g. Anaplasma · CPC title