Method for producing EPA-enriched oil and DHA-enriched oil

Patent metadata

Field	Value
Publication number	US-9556401-B2
Application number	US-66980308-A
Country	US
Kind code	B2
Filing date	Jul 29, 2008
Priority date	Jul 30, 2007
Publication date	Jan 31, 2017
Grant date	Jan 31, 2017

Abstract

Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.

First claim

The invention claimed is:

1. A method for producing eicosapentaenoic acid-enriched oil and docosahexaenoic acid-enriched oil from oils and fats containing eicosapentaenoic acid and docosahexaenoic acid as constituent fatty acids, said method comprising:
a) a step wherein said oils and fats are subjected to alcoholysis accompanied by hydrolysis, by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of an aqueous alcohol and magnesium oxide as a reaction additive, and an eicosapentaenoic acid and docosahexaenoic acid-enriched glyceride fraction is obtained from the resulting reaction mixture,
wherein water is added in the amount of 5% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol, and
b) a step wherein said glyceride fraction is subjected to alcoholysis accompanied by hydrolysis, by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of an aqueous alcohol and magnesium oxide as a reaction additive, and the eicosapentaenoic acid-enriched ester fraction and docosahexaenoic acid-enriched glyceride fraction are separated from the resulting reaction mixture,
wherein water is added in the amount of 5% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol;
wherein the lipase used in step a) is a lipase obtained from a microorganism belonging to Alcaligenes sp. and the lipase used in step b) is a lipase obtained from a microorganism belonging to Thermomyces lanuginosus;
and wherein the oils and fats used in step a) are fish oil.

2. The method according to claim 1 , wherein the lipase used in step a) is Lipase QLM®.

3. The method according to claim 1 , wherein the lipase used in step b) is Lipozyme TL IM®.

4. The method according to claim 1 , wherein the amount of reaction additive added in step a) and/or step b) is 0.01 to 30% (w/w) in terms of oils and fats.

5. The method according to claim 1 , wherein the alcohol is a lower alcohol selected from the group consisting of ethanol, methanol, 2-propanol, and butanol.

6. The method according to claim 1 , wherein in step (a) water is added in the amount of 10% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol.

7. The method according to claim 1 , wherein in step (b) water is added in the amount of 5.78% (v/v) to 20% (v/v) in terms of amount of the aqueous alcohol.

Assignees

• Furihata Kiyomi
• Kawahara Hiroyuki
• Yamaguchi Hideaki
• Ikemoto Hideo
• Doisaki Nobushige
• Nippon Suisan Kaisha Ltd

Inventors

• Furihata Kiyomi
• Kawahara Hiroyuki
• Yamaguchi Hideaki
• Ikemoto Hideo
• Doisaki Nobushige

Classifications

• C12P7/6472

  containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone · CPC title

• C11C1/045 Primary

  using enzymes or microorganisms, living or dead · CPC title

• C11C3/10

  Ester interchange · CPC title

• A23D9/02 Primary

  characterised by the production or working-up · CPC title

• C12P7/64

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title