Acyltransferases and uses thereof in fatty acid production

Patent metadata

Field	Value
Publication number	US-10208270-B2
Application number	US-201615159931-A
Country	US
Kind code	B2
Filing date	May 20, 2016
Priority date	Jun 25, 2010
Publication date	Feb 19, 2019
Grant date	Feb 19, 2019

Abstract

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

First claim

We claim:

1. A polynucleotide comprising an expression control sequence operatively linked to a heterologous nucleic acid sequence selected from the group consisting of:
a) the nucleic acid sequence of SEQ ID NO: 52 or 54;
b) a nucleic acid sequence encoding a diacylglycerol acyltransferase 2 (DGAT2) polypeptide comprising the amino acid sequence of SEQ ID NO: 53;
c) a nucleic acid sequence having at least 85% sequence identity to the nucleic acid sequence of SEQ ID NO: 52 or 54 and encoding a DGAT2 polypeptide, wherein said polypeptide has diacylglycerol acyltransferase activity; and
d) a nucleic acid sequence encoding a DGAT2 polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 53, wherein said polypeptide has diacylglycerol acyltransferase activity.

2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises a terminator sequence operatively linked to the nucleic acid sequence.

3. A vector comprising the polynucleotide of claim 1 .

4. A host cell comprising:
a) the polynucleotide of claim 1 ; or
b) a vector comprising said polynucleotide;
wherein the host cell is a plant cell, a microorganism, or an insect cell.

5. The host cell of claim 4 , wherein the host cell is a plant cell.

6. The host cell of claim 4 , wherein the host cell is yeast, fungus, algae, or moss.

7. A method for the manufacture of a polypeptide, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of said polypeptide; and
b) obtaining the polypeptide from said host cell.

8. A non-human transgenic organism comprising:
a) the polynucleotide of claim 1 ; or
b) a vector comprising said polynucleotide,
wherein the non-human transgenic organism is a plant or a microorganism.

9. The non-human transgenic organism of claim 8 , wherein the microorganism is a fungus, algae, moss, or yeast.

10. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell; and
b) obtaining said polyunsaturated fatty acids from said host cell.

11. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the non-human transgenic organism of claim 8 under conditions which allow for the production of polyunsaturated fatty acids in said host cell; and
b) obtaining said polyunsaturated fatty acids from said non-human transgenic organism.

12. The method of claim 11 , wherein said polyunsaturated fatty acid is arachidonic acid (ARA), eicosapentaenoic acid (EPA), and/or docosahexaenoic acid (DHA).

13. A method for the manufacture of an oil, lipid, or fatty acid composition, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell;
b) obtaining said polyunsaturated fatty acids from said host cell; and
c) formulating the polyunsaturated fatty acid as an oil, lipid, or fatty acid composition.

14. The method of claim 13 , wherein said oil, lipid, or fatty acid composition is to be used for feed, foodstuffs, cosmetics, or pharmaceuticals.

15. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining said polyunsaturated fatty acids from said plant or seeds thereof.

16. The method of claim 15 , wherein the polyunsaturated fatty acids are obtained from the seeds of said plant.

17. A method for the manufacture of an oil, lipid or fatty acid composition, comprising:
a) providing a polyunsaturated fatty acid produced by the method of claim 15 ; and
b) formulating said polyunsaturated fatty acid as an oil, lipid or fatty acid composition.

18. A method for the manufacture of an oil, lipid or tatty acid composition, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining an oil, lipid or fatty acid composition from said plant or seeds thereof.

19. The method of claim 18 , wherein the oil, lipid or fatty acid composition is obtained from the seeds of said plant.

20. A method for the production of feed, foodstuffs, cosmetics or pharmaceuticals, comprising:
a) obtaining an oil, lipid or fatty acid composition produced by the method of claim 18 ; and
b) processing said oil, lipid or fatty acid composition to produce feed, foodstuffs, cosmetics or pharmaceuticals.

21. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) obtaining an oil, lipid or fatty acid composition produced by the method of claim 18 ; and
b) obtaining polyunsaturated fatty acids from said oil, lipid or fatty acid composition.

22. A plant, or a plant part, plant cell, or seed thereof, wherein said plant, or said plant part, plant cell, or seed thereof comprises:
a) the polynucleotide of claim 1 ; or
b) a vector comprising said polynucleotide.

23. The polynucleotide of claim 1 , wherein said heterologous nucleic acid sequence encodes a DGAT2 polypeptide having diacylglycerol acyltransferase activity and has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 53.

24. The polynucleotide of claim 1 , wherein said heterologous nucleic acid sequence encodes a polypeptide having diacylglycerol acyltransferase activity and has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 53.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Senger Toralf
• Marty Laurent
• Stymne Sten
• Lindberg Yilmaz Jenny
• Napier Johnathan A
• Sayanova Olga
• Haslam Richard
• Noemi Ruiz Lopez

Classifications

• C12Y203/0102

  Diacylglycerol O-acyltransferase (2.3.1.20) · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• A23L33/115

  Fatty acids or derivatives thereof; Fats or oils · CPC title

• A23V2250/1882

  Polyunsaturated fatty acids · CPC title

• A23L33/12

  Fatty acids or derivatives thereof · CPC title