Method for producing oil containing polyunsaturated fatty acid using lipase

Patent metadata

Field	Value
Publication number	US-10138502-B2
Application number	US-201615099899-A
Country	US
Kind code	B2
Filing date	Apr 15, 2016
Priority date	May 28, 2010
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

A method for lowering saturated fatty acid content, the method comprising concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25° C.

First claim

The invention claimed is:

1. A method for lowering saturated fatty acid content in a glyceride containing a polyunsaturated fatty acid, the method comprising:
concentrating the polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to hydrolyze the glyceride containing the polyunsaturated fatty acid, wherein the glyceride containing the polyunsaturated fatty acid is a marine animal oil glyceride or a microorganism oil glyceride;
wherein the polyunsaturated fatty acid is a fatty acid having at least 20 carbon atoms and having at least 3 double bonds,
wherein the hydrolysis by the lipase is performed in the presence of 10 to 200 weight percent of water based on a total weight of the glyceride containing the polyunsaturated fatty acid, and at a temperature from 10° C. to 20° C.,
wherein the lipase is derived from a microorganism selected from the group consisting of Candida cylindracea and Alcaligenes sp.; and
wherein the saturated fatty acid content in the resulting glyceride is less than the saturated fatty acid content in the glyceride prior to the concentrating.

2. A method for preparing a marine animal oil glyceride containing a concentrated polyunsaturated fatty acid, the method comprising:
concentrating the polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to hydrolyze the marine animal oil glyceride containing the polyunsaturated fatty acid;
wherein content of saturated fatty acid in the resulting marine animal oil glyceride is not more than 12 area percent;
wherein the hydrolysis by the lipase is performed in the presence of 10 to 200 weight percent of water based on a total weight of the marine animal oil glyceride containing the polyunsaturated fatty acid, and at a temperature from 10° C. to 20° C.; and
wherein the lipase is derived from Candida cylindracea.

3. The method according to claim 2 , wherein content of docosahexaenoic acid in the resulting glyceride is not less than 40 area percent.

4. The method according to claim 2 , wherein content of docosahexaenoic acid in the resulting glyceride is not less than 46 area percent.

5. The method according to claim 2 , wherein content of saturated fatty acids in the resulting glyceride is not more than 10 area percent.

6. The method according to claim 2 , wherein content of palmitic acid in the resulting glyceride is not more than 8 area percent.

7. The method according to claim 2 , wherein content of palmitic acid in the resulting glyceride is not more than 6 area percent.

8. The method according to claim 2 , wherein content of triglyceride in the resulting glyceride is not less than 80 area percent.

9. The method according to claim 2 , wherein content of triglyceride in the resulting glyceride is not less than 85 area percent.

10. The method according to claim 1 , wherein the marine animal oil glyceride is derived from a fish, a crustacean, or a combination thereof.

11. The method according to claim 2 , wherein the marine animal oil glyceride is derived from a fish, a crustacean, or a combination thereof.

12. The method according to claim 1 , wherein the glyceride containing the polyunsaturated fatty acid is the microorganism oil glyceride produced by microorganisms belonging to the genera Mortierella, Penicillium, Aspergillus, Rhodotorula , or Fusarium.

13. The method according to claim 1 , wherein the glyceride containing the polyunsaturated fatty acid is the microorganism oil glyceride produced by Mortierella sp.

14. A method for preparing a microorganism oil glyceride containing a concentrated polyunsaturated fatty acid, the method comprising:
concentrating the polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to hydrolyze the microorganism oil glyceride containing the polyunsaturated fatty acid;
wherein the microorganism oil glyceride containing the polyunsaturated fatty acid is derived from Mortierella sp.;
wherein the hydrolysis by the lipase is performed in the presence of 10 to 200 weight percent of water based on a total weight of the microorganism oil glyceride containing the polyunsaturated fatty acid, and at a temperature from 10° C. to 20° C.;
wherein the lipase is derived from Candida cylindracea ; and
wherein saturated fatty acid content in the resulting glyceride is less than the saturated fatty acid content in the glyceride prior to the concentrating.

Assignees

• Nippon Suisan Kaisha Ltd

Inventors

• Ikemoto Hideo
• Doisaki Nobushige
• Umehara Yasuo

Classifications

• A23D9/013

  Other fatty acid esters, e.g. phosphatides · CPC title

• C11B3/001

  by a combination of two or more of the means hereafter · CPC title

• C11C1/045

  using enzymes or microorganisms, living or dead · CPC title

• Y02E50/17

  Cross-Sectional Technologies · mapped topic

• C12P7/6472 Primary

  containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone · CPC title