Protein adsorbent

Patent metadata

Field	Value
Publication number	US-9545627-B2
Application number	US-201214363919-A
Country	US
Kind code	B2
Filing date	Dec 12, 2012
Priority date	Dec 15, 2011
Publication date	Jan 17, 2017
Grant date	Jan 17, 2017

Abstract

A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.

First claim

The invention claimed is:

1. A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed to a surface of the substrate, the method comprising, in this order:
a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, the molecular chain comprising a weak electrolytic ion-exchange group; and
a wet-heat treatment step of heating the dry-heated adsorbent in a moistened state with a liquid selected from pure water, a buffer solution, an inorganic salt aqueous solution, and a buffer solution comprising an inorganic salt, or steam thereof, and
wherein the moistened state of the protein adsorbent is maintained after the wet-heat treatment step to obtain the protein adsorbent.

2. The method according to claim 1 , further comprising a molecular chain forming step of fixing the molecular chain on the surface of the substrate before the dry-heat treatment step.

3. The method according to claim 1 , wherein a dynamic adsorption capacity of bovine serum albumin or lysozyme of the protein adsorbent is 5 mg/mL or more.

4. The method according to claim 1 , wherein the dry-heat treatment step comprises heating the pretreatment adsorbent at 40° C. to 130° C. for 1 hour or more.

5. The method according to claim 1 , wherein the wet-heat treatment step comprises heating the pretreatment adsorbent at 40 to 120° C. for 30 minutes or more in the moistened state.

Assignees

• Asahi Kasei Chemicals Corp

Inventors

• Shinohara Naoyuki
• Sato Yuta

Classifications

• B01J41/13

  obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds · CPC title

• B01J41/07

  in the weakly basic form · CPC title

• B01J20/3217

  Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond · CPC title

• B01J20/28023

  Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence) · CPC title

• C07K1/22

  Affinity chromatography or related techniques based upon selective absorption processes · CPC title