Multi-color nanoscale imaging based on nanoparticle cathodoluminescence

Patent metadata

Field	Value
Publication number	US-9541512-B2
Application number	US-201214125934-A
Country	US
Kind code	B2
Filing date	Jun 13, 2012
Priority date	Jun 13, 2011
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

Multi-color CL images of nanoparticle samples may be generated, by irradiating with a scanning electron beam a nanoparticle sample that containing a plurality of spectrally distinct optical emitters configured to generate CL light at respective different color channels, then detecting the CL light from the nanoparticles to generate multi-color NP-CL images of the nanoparticle sample. In some embodiments, SE (secondary electron) images of the sample may be acquire, substantially simultaneously with the acquisition of the CL images, so as to generate correlative NP-CL and SE images of the nanoparticle sample. In some embodiments, the nanoparticles may be surface-functionalized so that the nanoparticles selectively bind only to particular structures of interest.

First claim

What is claimed is:

1. A system comprising:
an electron microscope configured to generate an image of a nanoparticle sample containing a plurality of nanoparticles, by irradiating the nanoparticle sample with electron beams;
wherein the nanoparticles comprise a plurality of spectrally distinct optical emitters configured to generate CL (cathodoluminescent) light at respective different color channels in response to the electron beams from the electron microscope; and
a CL detecting imaging system configured to detect the CL light from the nanoparticles and to generate multi-color CL images of the nanoparticle sample;
wherein the nanoparticles comprise nanodiamonds that contain NV (nitrogen-vacancy) color centers, and that are configured to produce red cathodoluminescent light at a wavelength of about 620 nm.

2. The system of claim 1 , wherein the nanoparticles further comprise LuAG:Ce (Cerium-doped Lutetium-Aluminum) nanophosphors that are configured to produce green cathodoluminescent light at a wavelength of about 510 nm.

3. The system of claim 1 , wherein the Nanoparticles further comprise nanodiamonds that include band-A defects and that are configured to produce blue cathodoluminescent light at a wavelength of about 420 nm.

4. The system of claim 1 , wherein the electron microscope and the CL imaging system are integrated into a single instrument.

5. The system of claim 1 , wherein the electron microscope comprises one of a SEM (scanning electron microscope); an STM (scanning tunneling microscope); an STEM (scanning transmission electron microscope) and a TEM (transmission electron microscope).

6. The system of claim 1 , wherein at least some of the nanoparticles are surface functionalized, so that each one of the surface-functionalized nanoparticles selectively binds only to a particular structure of interest.

7. The system of claim 6 , wherein the surface-functionalized nanoparticles comprise antibody-conjugated nanoparticles.

8. The system of claim 6 , wherein the particular structures of interest comprise synaptic vesicles.

Assignees

• Walsworth Ronald
• Lichtman Jeff
• Kasthuri Narayanan
• Glenn David
• Zhang Huiliang
• Schalek Richard
• Harvard College

Inventors

• Walsworth Ronald
• Lichtman Jeff
• Kasthuri Narayanan
• Glenn David
• Zhang Huiliang
• Schalek Richard

Classifications

• G01N2223/403

  mapping with false colours · CPC title

• H01J2237/225

  Displaying image using synthesised colours · CPC title

• H01J2237/2808

  Cathodoluminescence · CPC title

• G01N2223/612

  biological material · CPC title

• G01N2223/418

  electron microscope · CPC title