Apparatus for determining a vertical position of at least one interface between a first component and at least one second component and laboratory automation system

Patent metadata

Field	Value
Publication number	US-9534885-B2
Application number	US-201414175141-A
Country	US
Kind code	B2
Filing date	Feb 7, 2014
Priority date	Feb 21, 2013
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

An apparatus for determining a vertical position of an interface between a first component and a second component comprising different layers in a sample container comprises a first unit comprising a first emitting light, a first optics, and a first detector; a second unit vertically spaced from the first unit comprising a second emitting light, a second optics, and a second detector; a driving unit to move the first unit and the second unit relative to the sample container; a position sensing unit to output a position sensing signal indicative of a vertical position of the sample container; and a vertical position determining unit to calculate the vertical position of the interface.

First claim

We claim:

1. An apparatus for determining a vertical position of at least one interface between a first component and at least one second component comprised as different layers in a sample container, the apparatus comprising:
a first sensing unit comprising,
a first laser diode emitting light having a first wavelength transmitted by the sample container and the first component,
a first collimating optics to collimate the light having the first wavelength, such that the light is emitted in form of a beam having a defined diameter and direction in space, wherein the first collimating optics is adapted to the first wavelength, and
a first light detector generating a first sensing signal in response to an intensity of light having the first wavelength being applied to the first light detector;

a second sensing unit vertically spaced by a given vertical distance from the first sensing unit comprising,
a second laser diode emitting light having a second wavelength transmitted by the sample container but blocked by the first component,
a second collimating optics to collimate the light having the second wavelength such that the light is emitted in form of a beam having a defined diameter and direction in space, wherein the second collimating optics is adapted to the second wavelength, and wherein the resulting beam having the second wavelength and the resulting beam having the first wavelength propagate in parallel but vertically spaced paths separated by the given vertical distance, and
a second light detector generating a second sensing signal in response to an intensity of light having the second wavelength being applied to the second light detector;

a driving unit to move the sample container relative to the first sensing unit and the second sensing unit and to simultaneously insert the sample container into a sample container carrier parallel to detecting the vertical position of the at least one interface;
a position sensing unit to output a position sensing signal indicative of a vertical position of the sample container; and
a vertical position determining unit to match the first sensing signal and the second sensing signal such that first sensing signal and the second sensing signal correspond to identical vertical positions and to determine the vertical position of the at least one interface in response to the matched sensing signals and the position sensing signal.

2. The apparatus according to claim 1 , wherein the vertical position determining unit calculates the vertical position of the at least one interface using a ratio between the matched sensing signals.

3. The apparatus according to claim 1 , wherein the first and the second component are selected from a group comprising air, serum, separation gel or combinations thereof.

4. The apparatus according claim 1 , wherein the first wavelength ranges between 400 nm and 1200 nm.

5. The apparatus according claim 1 , wherein the second wavelength ranges between 1300 nm and 1700 nm.

6. The apparatus according to claim 1 , wherein the driving unit rotates the sample container around a vertical axis of the sample container.

7. The apparatus according to claim 6 , wherein the apparatus repeats the detecting of the vertical position of the at least one interface for the rotated sample container.

8. The apparatus according to claim 1 , further comprising,
a light barrier to detect the introduction of a sample container into the apparatus.

9. The apparatus according to claim 8 , wherein the apparatus activates the first and the second sensing unit when the introduction is detected.

10. A laboratory automation system for processing components comprised in a sample container, the system comprising:
the apparatus according to claim 1 ; and
at least one laboratory station coupled to the apparatus, wherein the at least one laboratory station operates in response to the detected vertical position of the at least one interface.

11. The system according to claim 10 , wherein at least one of the laboratory stations is an aliquoter unit having a pipetting unit having a tip, wherein during aliquoting the aliquoter unit controls a vertical position of the tip in response to the detected vertical position of the at least one interface.

12. The system according to claim 10 , further comprising,
a sample container transport unit to transport the sample container between different laboratory stations, wherein the sample container transport unit comprises a number of sample container carriers.

13. The system according to claim 12 , wherein the sample container transport unit comprises a conveyor.

14. The system according to claim 13 , wherein the sample container carriers are attached to the conveyor.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Klinec Darko
• Koehler Michael

Classifications

• G01N21/13

  Moving of cuvettes or solid samples to or from the investigating station {(handling materials for automatic analysis G01N35/00)} · CPC title

• G01F23/2921

  for discrete levels · CPC title

• G01N2035/1025

  Fluid level sensing · CPC title

• G01N33/48

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title

• G01N2035/0493

  Locating samples; identifying different tube sizes · CPC title