Apparatus and method for processing biological material

Patent metadata

Field	Value
Publication number	US-9423327-B2
Application number	US-201213616216-A
Country	US
Kind code	B2
Filing date	Sep 14, 2012
Priority date	Dec 1, 2008
Publication date	Aug 23, 2016
Grant date	Aug 23, 2016

Abstract

The application discloses an apparatus and method for processing biological material, including a suspension of cells.

First claim

The invention claimed is:

1. A method of concentrating or washing cells in a suspension, comprising:
a. collecting a suspension including a cell population within a first chamber that is in fluid flow connection with a closed second chamber, the second chamber only in fluid flow connection with the first chamber;
b. applying a centrifugal force field to the first chamber and the second chamber as a unit such that the centrifugal force field is applied to the cell population in the first chamber to obtain a concentrated cell suspension within the first chamber; and
c. flowing the concentrated cell suspension into the closed second chamber under the centrifugal force field, wherein when the centrifugal force field is applied to the first and second chambers as a unit the concentrated cell suspension will be transferred from the first chamber to the second chamber, wherein flowing the concentrated cell suspension into the closed second chamber includes flowing the concentrated cell suspension along a single linear flow axis that defines a common central axis of the first and second chambers.

2. The method of claim 1 further comprising disconnecting the first chamber from the second chamber and connecting the second chamber to a third chamber for further processing of the concentrated cell suspension.

3. The method of claim 1 wherein the steps of a-c are repeated but the suspension including a cell population that is collected in the first chamber when repeated is the concentrated cell suspension that was flowed to the second chamber.

4. A method of concentrating or washing cells in a suspension, comprising:
a. collecting a suspension including a cell population within a first chamber that is in fluid flow connection with a closed second chamber, the second chamber only in fluid flow connection with the first chamber;
b. applying a centrifugal force field to the first chamber and the second chamber as a unit such that the centrifugal force field is applied to the cell population in the first chamber to obtain a concentrated cell suspension within the first chamber;
c. flowing the concentrated cell suspension into the closed second chamber under the centrifugal force field, wherein when the centrifugal force field is applied to the first and second chambers as a unit the concentrated cell suspension will be transferred from the first chamber to the second chamber, wherein flowing the concentrated cell suspension into the closed second chamber includes flowing the concentrated cell suspension along a single linear flow axis that defines a common central axis of the first and second chambers;
d. disconnecting the second chamber from the first chamber; and
e. flowing the concentrated cell suspension into a further fluid destination or source, the further fluid destination or source adapted to be placed together with the second chamber in a sedimentation force field.

5. The method of claim 4 wherein the first and second chambers are adapted to be coupled together to form a sedimentation assembly which is adapted to be placed in a holder and subjected to centrifugation.

6. The method of claim 4 wherein the first chamber receives the suspension from a system for isolating cells.

7. The method of claim 4 wherein the suspension includes stem cells that have been isolated according to the presence or absence of one or more selected cell markers.

8. The method of claim 4 wherein the further destination or source contains a solution for washing the cells within the suspension.

9. The method of claim 4 wherein the further destination or source contains a solution for treating the cells within the suspension.

10. The method of claim 1 in which the first and second chambers are adapted to be coupled together to form a sedimentation assembly which is adapted to be placed in a holder and subjected to centrifugation.

11. The method of claim 1 in which the first chamber receives the suspension from a system for isolating cells.

12. The method of claim 1 in which the cell population has been isolated according to the presence or absence of one or more selected cell markers.

13. A method of concentrating or washing cells in a suspension, comprising:
a. collecting a suspension including a cell population within a closed first chamber including a cell concentration zone, the closed first chamber in fluid flow connection with a closed second chamber, the second chamber only in fluid flow connection with the first chamber;
b. applying a centrifugal force field to the first chamber and the second chamber as a unit such that the centrifugal force field is applied to the cell population in the first chamber to obtain a concentrated cell suspension within the cell concentration zone of the first chamber;
c. flowing the concentrated cell suspension into the second chamber under the centrifugal force field, wherein when the centrifugal force field is applied to the first and second chambers as a unit the concentrated cell suspension will be transferred from the first chamber to the second chamber, wherein flowing the concentrated cell suspension into the closed second chamber includes flowing the concentrated cell suspension along a single linear flow axis that defines a common central axis of the first and second chambers;
d. disconnecting the second chamber from the first chamber; and
e. flowing the concentrated cell suspension from the second chamber into a further fluid destination or source, the further fluid destination or source being in fluid flow connection with the second chamber and configured to be placed together with the second chamber in a sedimentation force field.

14. The method of claim 13 wherein the first chamber receives the suspension from a system for isolating cells.

15. The method of claim 13 wherein the suspension includes stem cells that have been isolated according to the presence or absence of one or more selected cell markers.

16. The method of claim 13 wherein the further destination or source contains a solution for washing or treating the cells within the suspension.

Assignees

• Min Kyungyoon
• Dudar Thomas E
• Laird James C
• Baxalta GmbH
• Baxalta Inc

Inventors

• Min Kyungyoon
• Dudar Thomas E
• Laird James C

Classifications

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• A61M39/045

  pre-slit to be pierced by blunt instrument · CPC title

• G01N1/40

  Concentrating samples · CPC title

• G01N2035/1058

  for mixing · CPC title

• B01D21/262

  by using a centrifuge · CPC title