Media for culturing stem cells
US-2016319241-A1 · Nov 3, 2016 · US
Method of enhancing cell growth using alkyl-amine-N-oxide (AANOx)
US9464269B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9464269-B2 |
| Application number | US-201514717346-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 20, 2015 |
| Priority date | Dec 30, 2008 |
| Publication date | Oct 11, 2016 |
| Grant date | Oct 11, 2016 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention relates to a method to enhance cell growth in culture comprising adding an alkyl-amine-n-oxide (AANOx), such as dodecyldimethylamine oxide (DDAO), into the culture medium in an amount sufficient to improve cell growth.
First claim
Opening claim text (preview).
What is claimed is: 1. A eukaryotic cell culture media comprising dodecyldimethylamine oxide (DDAO) or a related analyte in an amount sufficient to enhance cell growth when used in the culture media, wherein the analyte is selected from the group consisting of C10 dimethylamine oxide, C12 dimethyl amine oxide, C14 dimethyl amine oxide, C16 dimethylamine oxide, C18 dimethylamine oxide, dimethyl-tetradecyl-amine-oxide and dimethyl-hexadecyl-amine-oxide. 2. The media of claim 1 wherein the amount of DDAO or the related analyte is between about 4 and about 80 ppb. 3. The media of claim 1 wherein the amount of DDAO or the related analyte is between about 4 and about 50 ppb. 4. The media of claim 1 wherein the amount of DDAO or the related analyte is between about 10 ppb and about 40 ppb. 5. The media of claim 1 , wherein the culture media is animal protein-free media. 6. The media of claim 1 , wherein the culture media comprises animal protein. 7. The media of claim 1 wherein the cells are mammalian cells. 8. The media of claim 1 , wherein the cells are selected from the group consisting of BSC cells, LLC-MK cells, CV-1 cells, COS cells, VERO cells, MDBK cells, MDCK cells, CRFK cells, RAF cells, RK cells, TCMK-1 cells, LLCPK cells, PK15 cells, LLC-RK cells, MDOK cells, BHK-21 cells, CHO cells, NS-1 cells, MRC-5 cells, WI-38 cells, BHK cells, 293 cells, and RK cells.
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Classifications
Amines, e.g. putrescine · CPC title
Serum-free medium, which may still contain naturally-sourced components · CPC title
- C12N5/0043Primary
Medium free of human- or animal-derived components · CPC title
from plants · CPC title
Serum-free culture media · CPC title
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Frequently asked questions
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- What does patent US9464269B2 cover?
- The present invention relates to a method to enhance cell growth in culture comprising adding an alkyl-amine-n-oxide (AANOx), such as dodecyldimethylamine oxide (DDAO), into the culture medium in an amount sufficient to improve cell growth.
- Who is the assignee on this patent?
- Baxalta Inc, Baxalta GmbH
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0043. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 11 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).