Dry granulated cell culture media

Patent metadata

Field	Value
Publication number	US-9399757-B2
Application number	US-201113994239-A
Country	US
Kind code	B2
Filing date	Nov 18, 2011
Priority date	Dec 16, 2010
Publication date	Jul 26, 2016
Grant date	Jul 26, 2016

Abstract

The present invention relates dry granulated cell culture media which do not comprise peptones or tryptones, especially to dry granulated cell culture media formulations that support the growth of mammalian, and/or insect and/or plant cells. The present invention further relates to the production of these dry granulated cell culture media and their use.

First claim

The invention claimed is:

1. A method for producing a dry granulated cell culture medium which does not comprise peptones or tryptones comprising:
a) providing a cell culture medium in the form of a mixed powder of the medium components, whereby the medium does not comprise peptones or tryptones; and
b) filling said mixed powder in a roll press and compacting said mixed powder in said roll press,

wherein the method is performed without the addition of water or other solvents.

2. A method according to claim 1 , wherein the cell culture medium that is provided in step a) is a mammalian cell culture medium.

3. A method according to claim 1 , wherein the cell culture medium that is provided in step a) comprises one or more saccharide components, one or more amino acids, one or more vitamins, one or more salts, one or more buffer components, one or more co-factors and one or more nucleic acid components.

4. A method according to claim 1 , wherein step b) comprises:
b1) compacting said mixed powder in a roll press; and
b2) reintroducing all parts of the compacted cell culture medium obtained in step b1) which have a particle size of smaller than 0.5 mm into the cell culture medium in the form of a mixed powder of the medium components to be filled in the roll press.

5. A method according to claim 1 , wherein the press capacity of the roll press is between 30 and 80 kN/cm roll width.

6. A method according to claim 1 , wherein the size of the gap between the rolls of the roll press is between 0.5 and 3 mm.

7. A method according to claim 1 , wherein in a step c) the material obtained from step b) is further processed in a sieve mill.

Assignees

• Rayner-Brandes Michael Howard
• Zhao Xiaojian (David)
• Merck Patent Gmbh

Inventors

• Rayner-Brandes Michael Howard
• Zhao Xiaojian (David)

Classifications

• C12N5/0043 Primary

  Medium free of human- or animal-derived components · CPC title

• C12N5/0018 Primary

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• C12N5/0602 Primary

  Vertebrate cells · CPC title

• C12N5/00

  Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) · CPC title