Antibiotic-free plasmids
US-9217153-B2 · Dec 22, 2015 · US
Method for culturing mammalian cells to improve recombinant protein production
US9388447B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9388447-B2 |
| Application number | US-201514821574-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 7, 2015 |
| Priority date | Apr 21, 2011 |
| Publication date | Jul 12, 2016 |
| Grant date | Jul 12, 2016 |
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- Title
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Abstract
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The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.
First claim
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What is claimed is: 1. A method of culturing Chinese Hamster Ovary (CHO) cells expressing a recombinant protein, comprising growing the CHO cells in a defined serum-free culture medium during a growth phase and maintaining the CHO cells in the cell culture medium during a production phase by supplementing the cell culture with a concentrated serum-free defined feed medium containing tyrosine and further supplementing the cell culture with one or more independent tyrosine feeds, wherein the independent tyrosine feed provides at least about 1 mM to at least about 2 mM tyrosine at each feed, wherein viability was prolonged, specific productivity was maintained, and titer was improved compared to CHO cells not receiving independent tyrosine feeds. 2. The method according to claim 1 , wherein the independent tyrosine feed provides at least about 1 mM tyrosine. 3. The method according to claim 2 , wherein the independent tyrosine feed provides at least about 1.38 mM tyrosine. 4. The method according to claim 1 , wherein the concentration of tyrosine in the cell culture medium does not exceed 8 mM. 5. The method according to claim 1 , wherein the independent tyrosine feed begins at least by day 5 of the production phase. 6. The method according to claim 1 , wherein the independent tyrosine feed begins on day 3 of the production phase. 7. The method according to claim 1 , wherein the independent tyrosine feeds begin on day 7. 8. The method according to claim 1 , wherein the independent tyrosine feed begins prior to the production phase. 9. The method according to claim 1 , wherein the independent tyrosine feed is made concurrently with the feed of the concentrated serum-free defined feed medium. 10. The method according to claim 1 , wherein the independent tyrosine feed is not concurrent with the feed of the concentrated serum-free defined feed medium. 11. The method according to claim 1 , wherein the recombinant protein is selected from the group consisting of a human antibody, a humanized antibody, a chimeric antibody, a recombinant fusion protein, or a cytokine.
Assignees
Inventors
Classifications
Amino acids · CPC title
- C12P21/00Primary
Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title
Cells for production · CPC title
Cells for large scale production · CPC title
- C12N5/0037Primary
Serum-free medium, which may still contain naturally-sourced components · CPC title
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Frequently asked questions
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- What does patent US9388447B2 cover?
- The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.
- Who is the assignee on this patent?
- Amgen Inc, Amgen Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P21/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 12 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).