Methods for producing bioeffective krill oil compositions

Patent metadata

Field	Value
Publication number	US-9375453-B2
Application number	US-201314020162-A
Country	US
Kind code	B2
Filing date	Sep 6, 2013
Priority date	Mar 28, 2007
Publication date	Jun 28, 2016
Grant date	Jun 28, 2016

Abstract

New krill oil compositions are disclosed as having high amounts of phospholipids, astaxanthin esters and/or omega-3 contents. The krill oils are obtained from krill meal using supercritical fluid extraction in a two stage process. Stage 1 removes the neutral lipid by extracting with neat supercritical CO 2 or CO 2 plus approximately 5% of a co-solvent. Stage 2 extracts the actual krill oils by using supercritical CO 2 in combination with approximately 20% ethanol. The krill oil materials obatined are compared with commercially available krill oil and found to be more bioeffective in a number of areas such as anti-inflammation, anti-oxidant effects, improving insulin resistances and improving blood lipid profile.

First claim

The invention claimed is:

1. A method of production of polar krill oil from Euphausia superba comprising:
a) treating the Euphausia superba to denature lipases and phospholipases to provide a denatured krill product;
b) contacting the denatured krill product with a polar solvent to extract a polar krill oil comprising phospholipids, said polar krill oil comprises greater than about 3% ether phospholipids w/w of said polar krill oil; from about 27% to 50% non-ether phospholipids w/w of said polar krill oil so that the amount of total phospholipids is from about 30% to 60% w/w of said polar krill oil; from about 20% to 50% triglycerides w/w of said polar krill oil, and astaxanthin esters in amount of greater than about 100 mg/kg of said polar krill oil; and
c) formulating said polar krill oil with a carrier for oral consumption.

2. The method of claim 1 , wherein said polar krill oil comprises greater than about 40% phosphatidylcholine w/w of said polar krill oil.

3. The method of claim 1 , wherein said polar krill oil comprises greater than about 45% phosphatidylcholine w/w of said polar krill oil.

4. The method of claim 1 , wherein said polar krill oil comprises at least 36% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

5. The method of claim 1 , wherein said method further comprises formulating said polar krill oil for oral consumption by a human.

6. The method of claim 5 , wherein said formulating comprises encapsulating said polar krill oil in a capsule.

7. The method of claim 1 , wherein said polar krill oil contains greater than about 200 mg/kg astaxanthin esters.

8. The method of claim 1 , wherein said polar krill oil contains greater than about 300 mg/kg astaxanthin esters.

9. The method of claim 1 , wherein said polar krill oil contains greater than about 400 mg/kg astaxanthin esters.

10. The method of claim 1 , wherein said polar krill oil comprises from about 20% to 35% omega-3 fatty acids as a percentage of total fatty acids in said composition polar krill oil.

11. The method of claim 10 , wherein from about 70% to 95% of said omega-3 fatty acids are attached to said phospholipids.

12. The method of claim 6 , wherein said polar krill oil is encapsulated in a soft gel capsule.

13. The method of claim 1 , further comprising combining said polar krill oil with a plant phytonutrient.

14. The method of claim 1 , wherein said polar krill oil comprises greater than about 4% w/w ether phospholipids.

15. The method of claim 14 , wherein said polar krill oil comprises greater than about 200 mg/kg astaxanthin esters.

16. The method of claim 14 , wherein said polar krill oil comprises greater than about 300 mg/kg astaxanthin esters.

17. The method of claim 15 , wherein said polar krill oil comprises from about 20% to 35% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

18. The method of claim 17 , wherein from about 70% to 95% of said omega-3 fatty acids are attached to said phospholipids.

19. The method of claim 14 , wherein said polar krill oil comprises greater than about 400 mg/kg astaxanthin esters.

20. The method of claim 19 , wherein said polar krill oil comprises from about 20% to 35% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

21. The method of claim 20 , wherein from about 70% to 95% of said omega-3 fatty acids are attached to said phospholipids.

22. The method of claim 16 , further comprising combining said polar krill oil with a plant phytonutrient.

23. The method of claim 1 , wherein said polar krill oil comprises greater than about 5% w/w ether phospholipids.

24. The method of claim 23 , wherein said polar krill oil comprises greater than about 200 mg/kg astaxanthin esters.

25. The method of claim 23 , wherein said polar krill oil comprises greater than about 300 mg/kg astaxanthin esters.

26. The method of claim 24 , wherein said polar krill oil comprises from about 20% to 35% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

27. The method of claim 26 , wherein from about 70% to 95% of said omega-3 fatty acids are attached to said phospholipids.

28. The method of claim 23 , wherein said polar krill oil comprises greater than about 400 mg/kg astaxanthin esters.

29. The method of claim 24 , further comprising combining said polar krill oil with a plant phytonutrient.

30. The method of claim 1 , wherein the treating step occurs on a ship.

31. The method of claim 1 , wherein said Euphausia superba is freshly caught.

32. The method of claim 1 , wherein said treating is a heat treatment.

33. A method of production of polar krill oil from Euphausia superba comprising:
a) treating the Euphausia superba to denature lipases and phospholipases to provide a denatured krill product;
b) contacting the denatured krill product with a polar solvent to extract a polar krill oil comprising phospholipids, and said polar krill oil comprises greater than about 3% ether phospholipids w/w of said polar krill oil; from about 27% to 50% non-ether phospholipids w/w of said polar krill oil so that the amount of total phospholipids is from about 30% to 60% w/w of said polar krill oil, from about 20% to 50% triglycerides w/w of said polar krill oil, and astaxanthin esters in amount of greater than about 100 mg/kg of said polar krill oil; and
c) encapsulating said polar krill oil in a soft gel capsule.

34. The method of claim 33 , wherein said Euphausia superba is freshly caught.

35. The method of claim 33 , wherein said treating is a heat treatment.

36. The method of claim 33 , wherein the treating step occurs on a ship.

37. The method of claim 33 , wherein said polar krill oil comprises greater than about 40% phosphatidylcholine w/w of said polar krill oil.

38. The method of claim 33 , wherein said polar krill oil comprises greater than about 45% phosphatidylcholine w/w of said polar krill oil.

39. The method of claim 33 , wherein said polar krill oil comprises at least 36% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

40. The method of claim 33 , wherein said polar krill oil contains greater than about 200 mg/kg astaxanthin esters.

41. The method of claim 33 , wherein said polar krill oil contains greater than about 300 mg/kg astaxanthin esters.

42. The method of claim 33 , wherein said polar krill oil contains greater than about 400 mg/kg astaxanthin esters.

43. The method of claim 33 , wherein said polar krill oil comprises from about 20% to 35% omega-3 fatty acids as a percentage of total fatty acids in said polar krill oil.

44. The method of claim 43 , wherein from about 70% to 95% of said omega-3 fatty acids are attached to said phospholipids.

45. The method of claim 33 , further comprising combining said polar krill oil with a plant phytonutrient.

46. The method of claim 33 , wherein said polar krill oil comprises greater than about 4% w/w ether phospholipids.

47. The method of claim 46 , wherein sa

Assignees

• Aker Biomarine Antarctic As

Inventors

• Bruheim Inge
• Tilseth Snorre
• Mancinelli Daniele

Classifications

• A61P3/02

  Nutrients, e.g. vitamins, minerals · CPC title

• A61K9/4858

  Organic compounds · CPC title

• A23L33/115

  Fatty acids or derivatives thereof; Fats or oils · CPC title

• A61K31/685

  one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin · CPC title

• A61K9/48

  Preparations in capsules, e.g. of gelatin, of chocolate {(A61K9/0004 takes precedence; bite capsules A61K9/0056)} · CPC title