Magnetic recovery method of magnetically responsive high-aspect ratio photoresist microstructures

Patent metadata

Field	Value
Publication number	US-9340417-B2
Application number	US-201213535296-A
Country	US
Kind code	B2
Filing date	Jun 27, 2012
Priority date	Jun 29, 2011
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

Systems and methods that facilitate magnetic collection and/or manipulation of individual micropallets are provided. The embodiments provided herein are directed to a new method for collecting micropallets once released from a substrate. It is accomplished by endowing the micropallets with magnetic properties by incorporating ferromagnetic or superparamagnetic nanoparticles into the photoresist material or otherwise incorporating magnetically responsive material into the micropallet structure. The magnetic particles, which posses magnetic qualities, e.g., ferromagnetism, ferrimagnetism, paramagnetism, and are composed of iron, nickel, and/or other magnetic materials, are mixed into the bulk photoresist prior to its use in the fabrication of microstructures. Also covered are other methods of incorporating magnetically-attractable material into the micropallet structure, such as plating of the micropallets with a material that is magnetically responsive, such as nickel. Additionally, the embodiments provided include a “collection probe” that is used to collect the released magnetic micropallets.

First claim

What is claimed is:

1. An assembly for addressable biochemical analysis comprising
an array of pallets releasably attached to a substrate and removable from the substrate upon application of an energy pulse applied along the substrate at the location of a pallet of interest, wherein each of the pallets comprises magnetically responsive material enabling each pallet to be manipulated by a magnetic field; and
a collection probe for generating the magnetic field to operably couple to the pallet of interest when removed from the substrate and configured to maintain a collected pallet of interest submerged in liquid, wherein the collection probe has a rod and a magnet attaching to the rod, wherein the magnet is detachable from the probe for collection of the magnet and the pallet coupled to the magnet, wherein the magnet is a permanent magnet that allows immediate generation and cessation of the magnetic field to attract and hold a pallet to collect the pallet of interest when removed from the substrate, wherein the permanent magnet is removable from the rod,
wherein the probe has a collection cavity that keeps a collected pallet of interest submerged in liquid throughout a transfer process.

2. The assembly of claim 1 wherein the magnetically responsive material comprises magnetic nano-particles or micro-particles.

3. The assembly of claim 2 wherein the magnetic particles comprise iron or nickel.

4. The assembly of claim 1 wherein the each of the pallets is formed from a ferromagnetic photopolymer.

5. A device for addressable biochemical analysis comprising
a plate having a top surface,
an array of pallets releasably attached to the top surface of the plate and dislodgeable from the top surface upon application of a force applied along the plate at the location of a pallet of interest, wherein each of the pallets comprises magnetically responsive material enabling each pallet to be manipulated by a magnetic field, and
a collection probe for generating the magnetic field to collect the pallet when released from the top surface of the plate and configured to maintain a collected pallet of interest submerged in liquid, wherein the collection probe has a rod and a magnet attaching to the rod, wherein the magnet is detachable from the probe for collection of the magnet and the pallet coupled to the magnet,
wherein the probe has a collection cavity that keeps the collected pallet of interest submerged in liquid throughout a transfer process.

6. The device of claim 5 wherein the magnetically responsive material comprises magnetic nano-particles.

7. The device of claim 6 wherein the magnetic particles comprise iron or nickel.

8. The device of claim 5 wherein the each of the pallets is formed from a ferromagnetic photopolymer.

9. The device of claim 5 wherein the force is an energy pulse.

10. The assembly of claim 5 wherein the magnet is an electro magnet.

Assignees

• Bachman Mark
• Nelson Edward
• Gunn Nicholas
• Univ California

Inventors

• Bachman Mark
• Nelson Edward
• Gunn Nicholas

Classifications

• C40B60/00 Primary

  Apparatus specially adapted for use in combinatorial chemistry or with libraries · CPC title

• C40B40/00

  Libraries per se, e.g. arrays, mixtures · CPC title

• B82Y15/00 Primary

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title