Method for dosing a liquid using a pipette and a syringe, and pipette for operating a syringe for dosing a liquid

Patent metadata

Field	Value
Publication number	US-9289762-B2
Application number	US-201313867800-A
Country	US
Kind code	B2
Filing date	Apr 22, 2013
Priority date	Apr 23, 2012
Publication date	Mar 22, 2016
Grant date	Mar 22, 2016

Abstract

A method for dosing a liquid using a pipette with adjustable dosing step size and an indicating equipment and a syringe that can be operated using the pipette. The syringe is detachably connected to the pipette. The dosing step size is set, or the dosing step size set before is maintained. The dosing volume adjusted via the dosing step size is indicated. Liquid is aspirated into the syringe. Dosing steps are performed, counted and the number of performed dosing steps and/or the number of dosing steps still possible without refilling the syringe is determined and indicated. After performing the maximum number of dosing steps possible without refilling the syringe, either the syringe is detached, or the steps are performed again. The overall performed number of dosing steps with the set dosing step size is counted, and/or the number of dosing steps still possible without refilling is determined and indicated.

First claim

The invention claimed is:

1. A pipette for handling a syringe, with
a bar-shaped housing ( 2 ),
a fixture ( 20 ) with an opening ( 21 . 1 ) at the lower end of the housing ( 2 ) for putting in a syringe ( 3 ) with a fastening portion ( 16 ) on the upper edge of a cylinder ( 11 ),
a fixture body ( 25 ) with a further fixture ( 24 ) and a further opening ( 21 . 2 ) on the lower end of the housing ( 2 ), for putting in a further fastening portion ( 19 ) of a piston ( 12 ) of the syringe ( 3 ),
means for detachably holding ( 23 , 26 ) the fastening portion ( 16 ) in the fixture ( 20 ) and the further fastening portion ( 19 ) in the further fixture ( 24 ),
means for relocating ( 5 , 8 , 34 , 37 , 27 ) the fixture body ( 25 ) within the housing ( 2 ) in the longitudinal direction of the housing ( 2 ),
which comprise a draw-up lever ( 5 ), activated from the outside of the housing ( 2 ) for picking up liquid into the syringe,
an actuation button ( 8 ), activated from the outside of the housing ( 2 ) for stepwise discharging liquid from the syringe ( 3 ),
an adjusting element ( 10 ), adjustable from the outside of the housing ( 2 ) for setting a dosing step size,
means for detecting ( 43 ) the dosing step size set by means of the adjusting element ( 10 ),
means for detecting ( 22 ) a syringe ( 3 ) that is detachably connected to the pipette ( 1 ),
means for detecting ( 84 ) the execution of a dosing step by actuating the actuation button ( 8 ),
an electronic control equipment ( 87 ), connected to the means for detecting ( 22 , 43 , 84 ) the dosing step size, the syringe and the execution of the dosing step,
an electronic indicating equipment ( 9 ), connected to the electronic control equipment ( 87 ),
wherein the control equipment ( 87 ) is configured such that upon syringe ( 3 ) inserted into the pipette ( 1 ), it determines the dosing volume based on the set dosing step size and the maximum possible number of dosing steps with the set dosing step size and completely filled syringe ( 3 ) without refilling the syringe ( 3 ), and indicates the dosing volume and maximum possible number of dosing steps by means of the indicating equipment, and determines the number of executed dosing steps and indicates the number of performed dosing steps and/or the number of still possible dosing steps without refilling the syringe by means of the indicating equipment ( 9 ).

2. The pipette according to claim 1 , which comprises a sensor for detecting an encoding imposing the syringe volume on a syringe ( 3 ) that is detachably connected to the pipette ( 1 ).

3. A pipette according to claim 1 , which comprises means for detecting ( 81 ) a relocation of the fixture body ( 25 ) into a lower end position ( 67 ) for detachably connecting the pipette ( 1 ) to a syringe ( 3 ), and means for detecting ( 67 ) a relocation of the fixture body ( 25 ) into an upper end position for completely filling a syringe ( 3 ), wherein these means for detecting are connected to the electronic control equipment ( 87 ).

4. A pipette according to claim 1 , which comprises means for detecting ( 67 ) a reverse stroke, which detect the actuation of a transmission element ( 69 ) for triggering the reverse stroke and are connected to the electronic control equipment ( 87 ).

5. The pipette according to claim 4 , wherein the transmission element ( 69 ) is coupled to the fixture body ( 25 ), so that it is taken along in the relocation of the fixture body ( 25 ) into the upper end position and is taken along in the opposite direction in the reverse stroke, and the means for detecting ( 67 ) the relocation of the fixture body ( 25 ) into the upper end position are the means for detecting ( 67 ) the reverse stroke at the same time.

6. A pipette for handling a syringe, with
a bar-shaped housing ( 2 ),
a fixture ( 20 ) with an opening ( 21 . 1 ) at the lower end of the housing ( 2 ) for putting in a syringe ( 3 ) with a fastening portion ( 16 ) on the upper edge of a cylinder ( 11 ),
a fixture body ( 25 ) with a further fixture ( 24 ) and a further opening ( 21 . 2 ) on the lower end of the housing ( 2 ), for putting in a further fastening portion ( 19 ) of a piston ( 12 ) of the syringe ( 3 ),
detachable holding device ( 23 , 26 ) for holding the fastening portion ( 16 ) in the fixture ( 20 ) and the further fastening portion ( 19 ) in the further fixture ( 24 ),
relocation device ( 5 , 8 , 34 , 37 , 27 ) for relocating the fixture body ( 25 ) within the housing ( 2 ) in the longitudinal direction of the housing ( 2 ),
which comprise a draw-up lever ( 5 ), activated from the outside of the housing ( 2 ) for picking up liquid into the syringe,
an actuation button ( 8 ), activated from the outside of the housing ( 2 ) for stepwise discharging liquid from the syringe ( 3 ),
an adjusting element ( 10 ), adjustable from the outside of the housing ( 2 ) for setting a dosing step size,
detection device ( 43 ) for detecting the dosing step size set by means of the adjusting element ( 10 ),
syringe detector ( 22 ) for detecting a syringe ( 3 ) that is detachably connected to the pipette ( 1 ),
dosing step detector for detecting ( 84 ) the execution of a dosing step by actuating the actuation button ( 8 ),
an electronic control equipment ( 87 ), connected to the means for detecting ( 22 , 43 , 84 ) the dosing step size, the syringe and the execution of the dosing step,
an electronic indicating equipment ( 9 ), connected to the electronic control equipment ( 87 ),
wherein the control equipment ( 87 ) is configured such that upon syringe ( 3 ) inserted into the pipette ( 1 ), it determines the dosing volume based on the set dosing step size and the maximum possible number of dosing steps with the set dosing step size and completely filled syringe ( 3 ) without refilling the syringe ( 3 ), and indicates the dosing volume and maximum possible number of dosing steps by means of the indicating equipment, and determines the number of executed dosing steps and indicates the number of performed dosing steps and/or the number of still possible dosing steps without refilling the syringe by means of the indicating equipment ( 9 ).

7. The pipette according to claim 6 , which comprises a sensor for detecting an encoding imposing the syringe volume on a syringe that is detachably connected to the pipette.

Assignees

• Eppendorf Ag

Inventors

• Belgardt Herbert
• Molitor Peter
• Reichmuth Burkhardt

Classifications

• B01L3/0234 Primary

  Repeating pipettes, i.e. for dispensing multiple doses from a single charge · CPC title

• B01L2300/027

  Digital display, e.g. LCD, LED · CPC title

• B01L2300/02

  Identification, exchange or storage of information · CPC title

• B01L2200/023

  adapted for different sizes of tubes, tips or container · CPC title

• B01L3/0237 Primary

  Details of electronic control, e.g. relating to user interface · CPC title