Method for Dosing Liquid by Means of a Pipette and a Syringe, and Pipette for Actuating a Syringe for Dosing Liquid

Patent metadata

Field	Value
Publication number	US-2021001326-A1
Application number	US-202016934243-A
Country	US
Kind code	A1
Filing date	Jul 21, 2020
Priority date	Dec 16, 2016
Publication date	Jan 7, 2021
Grant date	—

Abstract

A pipette with an adjustable dosing increment, a display apparatus, a drawing lever to draw liquid into a syringe, and a control button to discharge liquid in steps from the syringe, and the syringe actuated by means of the pipette, wherein the syringe is releasably connected to the pipette, the dosing increment is optionally set, or the previously set dosing increment is retained, the dosing volume set by the dosing increment is displayed by means of the display apparatus, liquid is sucked into the syringe by actuating the drawing lever, the respective position of a drive element of a drive mechanism of the pipette for filling and draining the syringe when the drive element is shifted along a path is detected, the possible maximum number of dosing steps with the set dosing increment without refilling the syringe after executing the reverse stroke starting from the position of the drive element reached at the end of filling is determined and displayed by means of the display apparatus, after the syringe is completely or partially filled with liquid, the reverse stroke is executed by actuating the control button at least once, dosing steps are executed by actuating the dosing button, the executed dosing steps are counted, the number of executed dosing steps and/or the number of still possible dosing steps without refilling the syringe are determined and displayed by means of the display apparatus, and after executing the maximum possible number of dosing steps without refilling the syringe, either the syringe is removed from the pipette, or steps 1.2 to 1.8 are repeated with same syringe, wherein in step 1.8, the total number of dosing steps performed with the syringe with the set dosing increments is counted, and/or the number of still possible dosing steps without refilling the syringe is determined and displayed by means of the display apparatus.

First claim

1 . A pipette ( 1 ) for handling a syringe ( 3 ), comprising:
a strip-shaped housing ( 2 ), a seat ( 20 ) with an opening ( 21 . 1 ) at the bottom end of the housing ( 2 ) for inserting a syringe ( 3 ) with a fastening section ( 16 ) at the top edge of a cylinder ( 11 ), a seat body ( 25 ) with another seat ( 24 ) and another opening ( 21 . 2 ) at the bottom end in the housing ( 2 ) for inserting another fastening section ( 19 ) of a plunger ( 12 ) of the syringe ( 3 ), means for releasably holding the fastening section ( 16 ) in the seat ( 20 ) and the additional fastening section ( 19 ) in the additional seat ( 24 ), at least one means for displacing the seat body ( 25 ) within the housing ( 2 ) in the longitudinal direction of the housing ( 2 ), that have the drawing lever ( 5 ) that can be actuated outside of the housing ( 2 ) for drawing liquid into the syringe ( 3 ), a control button ( 8 ) that can be actuated outside of the housing ( 2 ) for the stepwise discharging of liquid from the syringe ( 3 ), and an adjusting element for adjusting a dosing increment that can be adjusted outside of the housing ( 2 ), means for detecting the dosing increment set with the adjusting element, means for detecting a syringe ( 3 ) releasably connected to the pipette ( 1 ), means for detecting the respective position of the means for displacing the seat body ( 25 ) when displacing the displacement means along a path relative to the housing ( 2 ), an electronic control apparatus ( 42 ) connected to the detection means, and an electronic display apparatus ( 9 ) connected to the electronic control apparatus ( 42 ), wherein the control apparatus ( 42 ) is configured so that it determines the dosing volume by the set dosing increment when the syringe ( 3 ) is inserted in the pipette ( 1 ), and determines the maximum possible number of dosing steps following the execution of the reverse stroke without refilling the syringe ( 3 ) given the set dosing increment and completely or partially filled syringe ( 3 ) by the position of the displacement means on the path, and displays them by the display apparatus ( 9 ), it determines the execution of a reverse stroke to be executed by at least once actuating the control button ( 8 ), and outputs output indicating this by means of the display apparatus ( 9 ) or another display apparatus until the reverse stroke is executed and/or after execution of the reverse stroke, and it determines the number of executed dosing steps and displays the number of executed dosing steps and/or the number of dosing steps still possible without refilling the syringe ( 3 ) by means of the display apparatus ( 9 ).

2 . The pipette ( 1 ) according to claim 1 , wherein the means for detecting the respective position of the displacement means has an incremental encoder arranged on the displacement element of the displacement means and at least one sensor ( 44 ) fixedly arranged in the housing ( 2 ) and connected to the control apparatus ( 42 ) for detecting the displacement of the incremental encoder.

3 . The pipette ( 1 ) according to claim 2 , wherein the displacement element is a toothed rack ( 27 ) whose bottom end is connected to the drawing body ( 25 ) that can be engaged with a pawl ( 37 ) pivotably mounted on the control button ( 8 ) when the control button ( 8 ) is actuated to displace the toothed rack ( 27 ) a dosing step upon actuation.

4 . The pipette ( 1 ) according to claim 2 , wherein the incremental encoder is designed as a single piece with the toothed rack ( 27 ), or is a component securely connected to the toothed rack ( 27 ).

Assignees

• Eppendorf Ag

Inventors

• Reichmuth Burkhardt

Classifications

• B01L2300/025

  Displaying results or values with integrated means · CPC title

• B01L2300/027

  Digital display, e.g. LCD, LED · CPC title

• B01L2400/0478

  pistons · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• B01L2200/0605

  Metering of fluids · CPC title