Method for dosing a liquid using a pipette and a syringe, and pipette for operating a syringe for dosing a liquid

Patent metadata

Field	Value
Publication number	US-10906034-B2
Application number	US-201615043865-A
Country	US
Kind code	B2
Filing date	Feb 15, 2016
Priority date	Apr 23, 2012
Publication date	Feb 2, 2021
Grant date	Feb 2, 2021

Abstract

A method for dosing a liquid using a pipette with adjustable dosing step size and an indicating equipment and a syringe that can be operated using the pipette, wherein the syringe is detachably connected to the pipette, at option, the dosing step size is set, or the dosing step size set before is maintained, the dosing volume adjusted via the dosing step size is indicated by means of the indicating equipment, the maximum number of dosing steps possible without refilling the syringe, with the set dosing step size and completely filled syringe is indicated by means of the indicating equipment, liquid is aspirated into the syringe, a reverse stroke is performed after the syringe is filled with liquid, dosing steps are performed, the performed dosing steps are counted and the number of performed dosing steps and/or the number of dosing steps still possible without refilling the syringe is determined and indicated by means of the indicating equipment, after performing the maximum number of dosing steps possible without refilling the syringe, either the syringe is detached from the pipette, or the previous steps are performed anew by means of the same syringe, wherein in the dosing step, the overall performed number of dosing steps with the syringe with the set dosing step size is counted, and/or the number of dosing steps still possible without refilling the syringe is determined and indicated by means of the indicating equipment.

First claim

The invention claimed is:

1. A method for dosing a liquid using a manually operated pipette with adjustable dosing step size and an indicating equipment, which is part of the pipette, and a syringe that can be operated using the pipette, wherein
the syringe is detachably connected to the pipette whereby a fixture body of the pipette fastens a portion of a piston of the syringe, and further wherein the fixture body is a hollow-cylindrical fixture body which has a further axially oriented opening for inserting and gripping levers for holding a fastening portion for fastening the portion of the piston of the syringe,
the dosing step size is set, or the dosing step size set before is maintained,
the dosing volume adjusted via the dosing step size is indicated by means of the indicating equipment, the maximum number of dosing steps possible without refilling the syringe, with the set dosing step size and completely filled syringe, is indicated by means of the indicating equipment through a control equipment,
liquid is aspirated into the syringe by manually actuating a pick up lever of the pipette which dislocates the fixture body within a housing of the pipette from a lower end position to an upper end position, the lower end position and the upper end position being end positions of the dislocation of the fixture body, and
a reverse stroke of the fixture body and the piston is performed by manually actuating an actuation button of the pipette after the syringe is filled with liquid before dosing steps are performed,
the fixture body cooperating with a lower detection means in the lower position thereof indicating that the fixture body is located in the lower end position, and an upper detection means detecting an execution of reverse stroke,
wherein when liquid is being aspirated into the syringe, the fixture body is dislocated from the lower detection means which sends a signal to the indicating equipment for an indication by blinking until the fixture body activates the upper detection means and when a reverse stroke is executed thereafter, the upper detection means are actuated by the actuation button and send a signal to the indicating equipment for blinking as long as a reverse stroke has been performed,
dosing steps are performed by manually actuating the actuation button, after the reverse stroke is performed, the performed dosing steps are counted and a number of performed dosing steps and/or a number of dosing steps still possible without refilling the syringe is determined and indicated by means of the indicating equipment, after performing the maximum number of dosing steps possible without refilling the syringe, either the syringe is detached from the pipette, or the previous steps are performed anew by means of the same syringe, wherein in the dosing steps, the overall performed number of dosing steps with the syringe with the set dosing step size is counted, and/or the number of dosing steps still possible without refilling the syringe is determined and indicated by means of the indicating equipment.

2. The method according to claim 1 , wherein an encoding specifying a syringe volume of the syringe that is connected to the pipette is detected by the pipette, and the dosing volume is determined on the basis of the set dosing step size and the detected encoding, and is indicated by means of the indicating equipment.

3. The method according to claim 1 , wherein an indication referring to the pick-up of liquid is indicated by means of the indicating equipment before liquid is picked up using the syringe that is detachably connected to the pipette.

4. The method according to claim 1 , wherein while liquid is being picked up, an indication, blinking or in other ways referring to the actual process of picking up liquid into the syringe and to the execution of the reverse stroke, is indicated by means of the indicating equipment for so long until a reverse stroke has been performed.

5. The method according to claim 1 , wherein after the complete execution of the reverse stroke, the set dosing volume and the number of performed dosing steps is indicated by means of the indicating equipment.

6. The method according to claim 5 , wherein an indication referring to the delivery of liquid is indicated after the complete execution of the reverse stroke.

7. The method according to claim 1 , wherein after performing the maximum possible number of dosing steps, an indication referring to the optional discharge of residual liquid from the syringe and to detaching the syringe from the pipette or to picking up liquid using the same syringe is indicated by means of the indicating equipment.

8. The method according to claim 7 , wherein after discharging residual liquid from the syringe, an indication referring to detaching the syringe from the pipette is indicated by means of the indicating equipment.

9. The method according to claim 1 , wherein the indication is indicated by means of the indicating equipment only after the syringe is detachably connected to the pipette, and wherein the indication expires when the syringe is being detached from the pipette.

10. The method according to claim 1 , wherein after detachably connecting the pipette to the syringe and before picking up liquid into the syringe, an indication referring to positioning the pipette for picking up liquid is indicated by means of the indicating equipment.

11. The method according to claim 1 , wherein after the execution of at least one dosing step and before the execution of the maximum possible number of dosing steps, changing the setting of the dosing step size, the newly set dosing volume and the maximum possible number of further dosing steps with the newly set dosing volume without refilling the syringe are indicated by means of the indicating equipment, and the executed number of further dosing steps with the newly set dosing volume is counted and indicated by means of the indicating equipment.

12. The method according to claim 11 , wherein after changing the dosing step size, the dosing step size is subsequently reset to the value set before, the maximum possible number of further dosing steps with the dosing step size set before and without refilling the syringe is indicated by means of the indicating equipment, and the dosing steps executed by means of the newly set dosing step size after the resetting, or the overall performed dosing steps before and after the resetting, are counted and indicated by means of the indicating equipment.

13. The method according to claim 1 , wherein an indication referring to the execution of a reverse stroke is indicated by means of the indicating equipment, when performing dosing steps after incomplete pick-up of liquid into the syringe, for so long until the reverse stroke is executed by one or several dosing steps, and dosing steps subsequent to this are counted and indicated by means of the indicating equipment.

14. A method for dosing a liquid comprising the steps of:
providing a pipette with adjustable dosing step size, the pipette having an attached indicating equipment;
providing a syringe that can be operated using the pipette;
detachably connecting the syringe to the pipette;
setting the dosing step size, or the dosing step size set before is maintained;
the dosing volume adjusted via the dosing step size is indicated using the indicating equipment;
the maximum number of dosing steps possible without refilling the syringe, with the set dosing step size and completely filled syringe, is indicated using the indicating equipment,
aspirating liquid into the syringe by manually actuating a pick up lever of the pipette which cooperates wi

Assignees

• Eppendorf Ag

Inventors

• Belgardt Herbert
• Molitor Peter
• Reichmuth Burkhardt

Classifications

• B01L2300/027

  Digital display, e.g. LCD, LED · CPC title

• B01L3/0237

  Details of electronic control, e.g. relating to user interface · CPC title

• B01L2200/06

  Fluid handling related problems · CPC title

• B01L2200/143

  Quality control, feedback systems · CPC title

• B01L2300/02

  Identification, exchange or storage of information · CPC title