Augmented reality personal assistant apparatus
US-9823737-B2 · Nov 21, 2017 · US
Chromatography equipment characterization
US9047438B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9047438-B2 |
| Application number | US-201313922101-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 19, 2013 |
| Priority date | Dec 21, 2010 |
| Publication date | Jun 2, 2015 |
| Grant date | Jun 2, 2015 |
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Abstract
Official abstract text for this publication.
Herein is reported a method for determining whether a re-usable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, comprising the following steps: a) identifying and determining the experimental data of an inert change of at least one physicochemical parameter of a mobile phase passing through said re-usable chromatography column packing, b) determining the parameters of a function of formula I by fitting the experimental data of the inert change of the physicochemical parameter of the at least second use, c) determining the difference between the experimental data of the inert change of the physicochemical parameter of the at least second use and the function of formula I with the parameters determined in step b), d) calculating the difference between the maximum value and the minimum value of the difference determined in step c) and normalizing said difference, e) determining reduced separation efficacy of said re-usable chromatography column packing when the absolute value of the difference calculated in step d) is more than 0.1.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for determining whether a re-useable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, characterized in that said method comprises the following steps: a) identifying and determining the experimental data of an inert change of at least one physicochemical parameter of a mobile phase passing through said re-useable chromatography column packing of the at least second use, b) determining the parameters of a function of formula I by fitting the experimental data of the inert change of the physicochemical parameter of the at least second use, c) determining the difference between the experimental data of the inert change of the physicochemical parameter of the at least second use and the function of formula I with the parameters determined in step b), d) calculating the difference between the maximum value and the minimum value of the difference determined in step c) and normalizing said difference, e) determining reduced separation efficacy of said re-useable chromatography column packing when the absolute value of the difference calculated in step d) is more than 0.1, wherein the function of formula I is yI = - 1 2 A · exp ( - x t 0 ) ( exp ( 2 x c t 0 + w 2 2 t 0 2 ) ( erf ( ( x - x c w - w t 0 ) 2 ) + 1 ) - exp ( x t 0 ) · erf ( x -
Assignees
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Classifications
Signal analysis · CPC title
Affinity chromatography or related techniques based upon selective absorption processes · CPC title
- G06F19/70Primary
Physics · mapped topic
for calibrating the measuring apparatus · CPC title
Conditioning of the sorbent material or stationary liquid · CPC title
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- What does patent US9047438B2 cover?
- Herein is reported a method for determining whether a re-usable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, comprising the following steps: a) identifying and determining the experimental data of an inert cha…
- Who is the assignee on this patent?
- Hoffmann La Roche, Hoffmann La Roche
- What technology area does this patent fall under?
- Primary CPC classification G06F19/70. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jun 02 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).