Enzyme preparations yielding a clean taste
US-2018177205-A1 · Jun 28, 2018 · US
US2025302077A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2025302077-A1 |
| Application number | US-202519232912-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jun 10, 2025 |
| Priority date | Dec 27, 2022 |
| Publication date | Oct 2, 2025 |
| Grant date | — |
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A reaction vessel includes a first vessel in which a working electrode having at least one of an enzyme or a coenzyme immobilized thereon is disposed, a second vessel in which a counter electrode is disposed, and a membrane that separates the first vessel and the second vessel from each other, prevents passage of an organic compound, and has ion conductivity. A food ingredient conversion method includes activating at least one of the enzyme or the coenzyme by applying a voltage between the working electrode and the counter electrode with an external power supply, transferring a proton between the organic compound and an external liquid by an enzymatic reaction using at least one of the activated enzyme or coenzyme, and performing ion conduction by transferring the proton in the external liquid through the membrane between the first vessel and the second vessel and preventing the organic compound from transferring.
Opening claim text (preview).
What is claimed is: 1 . A food ingredient conversion method comprising converting an organic compound in a reaction system including an external liquid and contained in a reaction vessel, wherein the reaction vessel includes a first vessel in which a working electrode having at least one of an enzyme or a coenzyme immobilized thereon is disposed, a second vessel in which a counter electrode is disposed, and a membrane that separates an inside of the first vessel and an inside of the second vessel from each other, prevents passage of the organic compound, and has ion conductivity, and the food ingredient conversion method includes activating at least one of the enzyme or the coenzyme by applying a voltage between the working electrode and the counter electrode with an external power supply located outside the reaction vessel and connected to the working electrode and the counter electrode, transferring a proton between the organic compound and the external liquid by an enzymatic reaction using at least one of the activated enzyme or coenzyme, and performing ion conduction by transferring the proton in the external liquid through the membrane between the first vessel and the second vessel and preventing the organic compound from transferring between the first vessel and the second vessel. 2 . The food ingredient conversion method according to claim 1 , wherein, in the transferring, the proton is transferred from the organic compound to the external liquid. 3 . The food ingredient conversion method according to claim 1 , wherein, in the transferring, the proton is transferred from the external liquid to the organic compound. 4 . The food ingredient conversion method according to claim 1 , wherein a redox coenzyme serving as the coenzyme is immobilized on the working electrode, and in the activating, an electron is transferred from the working electrode to the redox coenzyme, and the enzyme is activated by the redox coenzyme to which the electron has been transferred. 5 . The food ingredient conversion method according to claim 1 , wherein the coenzyme is a redox coenzyme, an electron mediator that transfers an electron to and from the redox coenzyme is further immobilized on the working electrode, and in the activating, an electron is transferred from the working electrode to the electron mediator, and at least one of the enzyme or the redox coenzyme is activated by the electron mediator to which the electron has been transferred. 6 . The food ingredient conversion method according to claim 4 , wherein the redox coenzyme is NADH or NADPH. 7 . The food ingredient conversion method according to claim 1 , wherein the organic compound includes at least one of a monosaccharide, a disaccharide, or a polysaccharide. 8 . The food ingredient conversion method according to claim 1 , wherein the organic compound includes an alcohol. 9 . The food ingredient conversion method according to claim 1 , wherein the organic compound has a disulfide bond. 10 . A food ingredient conversion device that converts an organic compound in a reaction system including an external liquid, the food ingredient conversion device comprising: a working electrode on which at least one of an enzyme or a coenzyme is immobilized; a counter electrode; a reaction vessel that contains the reaction system; and a voltage applicator that applies a voltage between the working electrode and the counter electrode, wherein the reaction vessel includes a first vessel in which the working electrode is disposed, a second vessel in which the counter electrode is disposed, and a membrane that separates an inside of the first vessel and an inside of the second vessel from each other, prevents passage of the organic compound, and has ion conductivity. 11 . The food ingredient conversion device according to claim 10 , wherein the enzyme is an oxidase. 12 . The food ingredient conversion device according to claim 10 , wherein the enzyme is a reductase. 13 . The food ingredient conversion device according to claim 10 , wherein the enzyme and the coenzyme are immobilized on the working electrode.
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