Methods and compositions for degrading cellulosic material
US-2015368685-A1 · Dec 24, 2015 · US
US9683224B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9683224-B2 |
| Application number | US-201214344508-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 14, 2012 |
| Priority date | Sep 14, 2011 |
| Publication date | Jun 20, 2017 |
| Grant date | Jun 20, 2017 |
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The present invention relates to new enzymes with improved properties and to compositions comprising these enzymes suitable for use in the production of a food, feed, or malt beverage product, such as in a brewing process.
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The invention claimed is: 1. A product selected from the group consisting of a food, a beverage, a feed, a paper product, a pulp product, and a biofuel wherein the product comprises: a first enzyme exhibiting endo-1,4-β-xylanase activity, said first enzyme having the amino acid sequence of SEQ ID NO:1; and a second enzyme exhibiting endo-1,3(4)-β-glucanase activity, said second enzyme having the amino acid sequence of SEQ ID NO:7. 2. The product according to claim 1 , further comprising at least one additional component chosen from the group consisting of an enzyme carrier, a stabilizer, and a preservative. 3. The product according to claim 1 , wherein the product has a xylanase activity from about 5000 U/g to about 8500 U/g. 4. The product according to claim 1 , wherein the product has a glucanase activity from about 10000 U/g to about 18000 U/g. 5. The according to claim 1 , wherein the product has a soluble arabinoxylan substrate to insoluble arabinoxylan substrate activity ratio (WE-AX/WU-AX) of less than about 7.0. 6. The product according to claim 1 , wherein the product has a temperature optimum from about 40° C. to about 70° C. 7. The product according to claim 1 , wherein the product comprises from about 200 to about 350 amino acids. 8. The product according to claim 1 , wherein at least one of the first enzyme and the second enzyme comprises a signal peptide. 9. A brewing mash comprising: a first enzyme exhibiting endo-1,4-β-xylanase activity, said first enzyme having the amino acid sequence of SEQ ID NO:1; a second enzyme exhibiting endo-1,3(4)-β-glucanase activity, said second enzyme having the amino acid sequence of SEQ ID NO:7; and starch.
acting on alpha -1,4-glucosidic bonds · CPC title
Cross-Sectional Technologies · mapped topic
acting on glycosyl compounds (3.2) · CPC title
Microorganisms or enzymes · CPC title
Endo-1,4-beta-xylanase (3.2.1.8) · CPC title
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