Low intensity vibration system and method for bioprocessing

Patent metadata

Field	Value
Publication number	US-2023203421-A1
Application number	US-202117928483-A
Country	US
Kind code	A1
Filing date	May 28, 2021
Priority date	May 29, 2020
Publication date	Jun 29, 2023
Grant date	—

Abstract

The present disclosure is directed to devices, systems and methods that include a stage and an actuator configured to transmit a orthogonal force to the stage, wherein the actuator is configured to receive a plurality of orthogonal acceleration signals, wherein the orthogonal acceleration signals comprise an actuator frequency signal and an actuator magnitude signal.

First claim

1 . A device comprising:
a stage; and an actuator configured to transmit a orthogonal force to the stage, wherein the actuator is configured to receive a plurality of orthogonal acceleration signals, wherein the orthogonal acceleration signals comprise an actuator frequency signal and an actuator magnitude signal.

2 . The device of claim 1 , wherein the actuator frequency signal is between about 0.1 Hz to about 1,000 Hz, or about 10 Hz to about 500 Hz, or about 20 Hz to about 150 Hz, or about 30 Hz to about 90 Hz, or about 30 Hz to about 35 Hz.

3 . The device of claim 1 , wherein the actuator magnitude signal is about 2 G’s or less, about 1.5 G’s or less, about 1.4 G’s or less, about 1.3 G’s or less, about 1.2 G’s or less, about 1.1 G’s or less, about 1 G or less, about 0.9 G’s or less, about 0.8 G’s or less, about 0.7 G’s or less, about 0.6 G’s or less, about 0.5 G’s or less, about 0.4 G’s or less, about 0.3 G’s or less, about 0.2 G’s or less, or about 0.1 G’s or less.

4 . The device of claim 1 , wherein the orthogonal acceleration signal further comprises one or more of a duration signal, a refractory period signal and a doses per time signal.

5 . The device of claim 1 , further comprising an accelerometer operably attached to the stage, wherein the accelerometer is configured to measure at least one of a stage frequency signal and a stage magnitude signal.

6 . The device of claim 5 , further comprising a processor configured to receive the stage frequency signal and the stage magnitude signal and configured to compare the stage frequency signal to the actuator frequency signal and configured to compare the stage magnitude signal to the actuator magnitude signal.

7 . The device of claim 6 , wherein the processor is further configured, if there is a difference between the stage frequency signal and the actuator frequency signal, and/or there is a difference between the stage magnitude signal and the actuator magnitude signal, to use the actuator frequency signal and/or the actuator magnitude signal as an input to a machine learning model that predicts whether or not a change to the plurality of orthogonal acceleration signals is to be made.

8 . The device of claim 7 , wherein the processor is further configured to transmit an updated actuator frequency signal and/or an updated actuator magnitude signal to the actuator based on the predicted change.

9 . The device of claim 8 , wherein the processor is further configured to access historical data and using the historical data as additional input to the machine learning model.

10 . The device of claim 6 , wherein if there is a difference between the stage frequency signal and the actuator frequency signal, the processor is configured to automatically transmit an updated actuator frequency signal to the actuator, and wherein if there is a difference between the stage magnitude signal and the actuator magnitude signal, the processor is configured to automatically transmit an updated actuator magnitude signal to the actuator.

11 . The device of claim 1 , further comprising a container supported by the stage, wherein the container comprises a liquid and a plurality of cells.

12 . The device of claim 11 , wherein the plurality of cells are selected from stem cells, T cells and combinations thereof.

13 . The device of claim 12 , wherein the stem cells are mesenchymal stem cells (MSCs).

14 . The device of claim 12 , wherein the T cells are selected from the group consisting of CD4+ T cells, CD8+ T cells, and CD3+ Pan T cells.

15 . The device of claim 11 , wherein the plurality of cells are suspended in the liquid, adhered to a surface, or both suspended in the liquid and adhered to the surface.

16 . The device of claim 15 , wherein the surface comprises a two-dimensional surface or a three-dimensional surface, wherein the two-dimensional surface or the three-dimensional surface is selected from the group consisting of an internal surface of the container, a particle within the container, and combinations thereof.

17 . The device of claim 11 , further comprising a processor configured to receive an updated concentration of the plurality of cells in the liquid at a time after the actuator receives a plurality of orthogonal acceleration signals and compare the received, updated concentration of the plurality of cells to a goal concentration of the plurality of cells.

18 . The device of claim 17 , wherein the processor is further configured if there is a difference between the updated concentration of the plurality of cells and the goal concentration, to use the plurality of orthogonal acceleration signals as an input to a machine learning model that predicts whether or not a change to the plurality of orthogonal acceleration signals is to be made.

19 . The device of claim 18 , wherein the processor is further configured to transmit one or more of an updated actuator frequency signal, an actuator magnitude signal, an updated duration signal, an updated refractory period signal and an updated doses per time signal to the actuator based on the predicted change.

20 . The device of claim 19 , wherein the processor is further configured to access historical data and using the historical data as additional input to the machine learning model.

21 . A method of proliferating cells, the method comprising:
contacting a stage of a device with a container, wherein the device comprises the stage and an actuator configured to transmit a orthogonal force to the stage, and wherein the container comprises a liquid and a plurality of cells; applying a orthogonal force from the actuator to the stage, wherein the actuator is configured to receive a plurality of the orthogonal acceleration signals, wherein the orthogonal acceleration signals comprise an actuator frequency signal and an actuator magnitude signal.

22 . The method of claim 21 , wherein the actuator frequency signal is between about 0.1 Hz to about 1,000 Hz, or about 10 Hz to about 500 Hz, or about 20 Hz to about 150 Hz, or about 30 Hz to about 90 Hz, or about 30 Hz to about 35 Hz.

23 . The method of claim 21 , wherein the actuator magnitude signal is about 2 G’s or less, about 1.5 G’s or less, about 1.4 G’s or less, about 1.3 G’s or less, about 1.2 G’s or less, about 1.1 G’s or less, about 1 G or less, about 0.9 G’s or less, about 0.8 G’s or less, about 0.7 G’s or less, about 0.6 G’s or less, about 0.5 G’s or less, about 0.4 G’s or less, about 0.3 G’s or less, about 0.2 G’s or less, or about 0.1 G’s or less.

24 . The method of claim 21 , wherein the orthogonal acceleration signal further comprises one or more of a duration signal, a refractory period signal and a doses per time signal.

25 . The method of claim 21 , wherein the device further comprises an accelerometer operably attached to the stage, wherein, during the applying the orthogonal force from the actuator to the stage step, the accelerometer measures at least one of a stage frequency signal and a stage magnitude signal.

26 . The method of claim 25 , wherein, during the applying the orthogonal force from the actuator to the stage step, the stage frequency signal and the stage magnitude signal are transmitted, and wherein the method further comprises the step of comparing the stage frequency signal to the actuator frequency signal and the step of comparing the stage magnitude signal to the actuator magnitude signal.

Assignees

• Univ New York State Res Found

Inventors

• Chan Mei Lin
• Rubin Clinton T

Classifications

• B01F31/24

  the containers being submitted to a rectilinear movement · CPC title

• B01F2215/0454

  Numerical frequency values · CPC title

• C12M27/16 Primary

  Vibrating; Shaking; Tilting · CPC title

• B01F35/222

  of the operation of the driving system, e.g. torque, speed or power of motors; of the position of mixing devices or elements · CPC title

• B01F35/2202

  Controlling the mixing process by feed-back, i.e. a measured parameter of the mixture is measured, compared with the set-value and the feed values are corrected · CPC title