Bioreactor with addition tube

Patent metadata

Field	Value
Publication number	US-2016194591-A1
Application number	US-201314910824-A
Country	US
Kind code	A1
Filing date	Aug 27, 2013
Priority date	Aug 27, 2013
Publication date	Jul 7, 2016
Grant date	—

Abstract

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

First claim

1 . A bioreactor comprising a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in said addition tube is located within said inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume and blocking flow in the reverse direction.

2 . The bioreactor of claim 1 , wherein the check valve is located at a distance from the delivery orifice, which distance is up to ten times, such as up to two times, the diameter of the addition tube.

3 . The bioreactor of claim 1 , wherein the delivery orifice of the addition tube is arranged to be immersed in liquid during use of the bioreactor.

4 . The bioreactor of claim 1 , wherein the delivery orifice is covered by the check valve.

5 . The bioreactor of claim 1 , wherein the opening pressure of said check valve is below or equal to 10 kPa, such as between 10 Pa and 10 kPa or between 100 Pa and 10 kPa.

6 . The bioreactor of claim 1 , wherein the check valve comprises an elastomeric closing member.

7 . The bioreactor of claim 6 , wherein the elastomeric closing member is selected from the group consisting of tubes, lips, flaps and split disks.

8 . The bioreactor of claim 1 , wherein the vessel comprises a flexible plastic bag.

9 . The bioreactor of claim 8 , wherein the flexible plastic bag is arranged to be supported by a rigid support structure during use.

10 . The bioreactor of claim 1 , wherein the agitation means comprises an impeller.

11 . The bioreactor of claim 8 , wherein the flexible plastic bag is inflatable and the agitation means comprises a movable support platform for the flexible plastic bag.

12 . The bioreactor of claim 1 , which is radiation sterilized.

13 . The bioreactor of claim 1 , wherein the addition tube is fluidically connected to a supply vessel containing a liquid solution comprising a pH regulator, a nutrient and/or a growth factor.

14 . The bioreactor of claim 1 , further comprising a liquid, such as a cell cultivation medium, in the inner volume of the vessel, and wherein the delivery orifice of the addition tube is immersed in said liquid.

15 . A method of cell cultivation, comprising the steps of:
a) providing a bioreactor according to claim 1 ; b) adding cell cultivation medium and cells to the inner volume; c) providing agitation by the agitation means, and d) adding a liquid solution and/or a gas to the inner volume via the addition tube.

16 . The method of claim 15 , wherein in step b) the amount of cell cultivation medium is sufficient to immerse the delivery orifice of the addition tube.

17 . The method of claim 15 , wherein the liquid solution comprises a pH regulator, a nutrient and/or a growth factor.

Assignees

• Ge Healthcare Bio Sciences Ab

Inventors

• Castan Andreas
• Gebauer Klaus

Classifications

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M29/00 Primary

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12N1/16

  Yeasts; Culture media therefor · CPC title

• C12N5/0601

  Invertebrate cells or tissues, e.g. insect cells; Culture media therefor · CPC title