System for automated explant preparation and method of use

Patent metadata

Field	Value
Publication number	US-2016376604-A1
Application number	US-201615195951-A
Country	US
Kind code	A1
Filing date	Jun 28, 2016
Priority date	Jun 29, 2015
Publication date	Dec 29, 2016
Grant date	—

Abstract

A system and method for the automated or semi-automated preparation of explants for transformation and transgenic engineering.

First claim

What is claimed is:

1 . A method for automated explant preparation, the method comprising:
operating a pump to fill an explant dish including a plurality of explants with an Agrobacterium solution, operating a first robotic arm to move the filled explant dish onto a shaker plate of a shaker station, operating the shaker station to move the shaker plate in a direction within a plane defined by the shaker plate to infect the plurality of explants with the Agrobacterium solution, and operating a second robotic arm to move an explant from the filled explant dish to a predetermined position on a cultivation media dish in response to determining the explant has been infected with the Agrobacterium solution.

2 . The method of claim 1 , further comprising operating the first robotic arm to move the cultivation media dish to the delivery station in response to determining the cultivation media dish has a predetermined number of explants positioned on the cultivation media dish.

3 . The method of claim 2 , wherein determining the cultivation media dish has the predetermined number of explants positioned on the cultivation media dish comprises determining the cultivation media dish has a number (n) of explants positioned on the cultivation media dish and the explants are evenly spaced 360/n degrees apart on the cultivation media dish.

4 . The method of claim 2 , wherein operating the first robotic arm to move the cultivation media dish comprises:
operating the first robotic arm to secure a lid of the cultivation media dish onto the cultivation media dish, and operating the first robotic arm to move the secured cultivation media dish to the delivery station.

5 . The method of claim 1 , further comprising:
capturing an image of a base of the filled explant dish with a camera, determining a location of an explant in the filled explant dish based on the image, and operating the second robotic arm to grip the explant at the location, wherein operating the second robotic arm to move the explant comprises operating the second robotic arm to move the explant in response to operating the second robotic arm to grip the explant.

6 . The method of claim 5 , wherein determining the location of the explant in the filled explant dish comprises:
determining locations of the plurality of explants in the filled explant dish, and selecting the explant from the plurality of explants.

7 . The method of claim 1 , further comprising selecting the cultivation media dish from a plurality of cultivation media dishes based on a number of explants currently positioned on each of the cultivation media dishes.

8 . The method of 7 , wherein selecting the cultivation media dish comprises selecting a cultivation media dish having fewer than six explants currently positioned on the cultivation media dish, and
wherein operating the second robotic arm to move the explant from the filled explant dish to the predetermined position on the selected cultivation media dish comprises determining a predetermined position on the selected cultivation media dish to which to move the explant based on a position of each other explant currently positioned on the cultivation media dish.

9 . The method of claim 1 , further comprising operating the first robotic arm to move each cultivation media dish of a plurality of cultivation media dishes from a dish dispenser to a predetermined position on a transfer station different from a position of each other cultivation media dish of the plurality of cultivation media dishes.

10 . The method of claim 9 , further comprising operating a second pump to pump the Agrobacterium solution from the filled explant dish and into a solution waste container in response to determining each cultivation media dish has a predetermined number of explants positioned on the cultivation media dish.

11 . The method of claim 10 , further comprising operating the first robotic arm to move the filled explant dish to a dish waste container in response to determining the Agrobacterium solution has been removed from the filled explant dish.

12 . The method of claim 1 , wherein operating the first robotic arm to move the filled explant dish comprises operating a claw grip of the first robotic arm with a compressed air source to grasp the filled explant dish, and
wherein operating the second robotic arm to move the explant comprises operating the second robotic arm to secure the explant with a suction force applied to the explant with a negative pressure source of the second robotic arm.

13 . The method of claim 1 , wherein operating the second robotic arm to move the explant comprises operating the second robotic arm to move the explant from the filled explant dish in response to determining that a desired infection time associated with infection of the explant has been reached.

14 . The method of claim 1 , wherein operating the shaker station to move the plate comprises moving the plate in movement pattern including at least one of rotational or side-to-side movements within the plane defined by the plate.

15 . The method of claim 1 , further comprising sterilizing a grip of the second robotic arm.

16 . The method of claim 1 , wherein the Agrobacterium solution comprises Agrobacterium tumefaciens.

17 . The method of claim 1 , wherein the Agrobacterium solution comprises Agrobacterium rhizogenes.

18 . An explant preparation apparatus, comprising:
a first robotic arm including a claw grip to grasp explant dishes for movement, a second robotic arm including a suction grip to secure explants with suction force for movement, a pump configured to deliver an Agrobacterium solution, a shaker station including a shaker plate and configured to move the shaker plate, and an electronic controller configured to: operate the pump to fill an explant dish including a plurality of explants with an Agrobacterium solution, operate the first robotic arm to move the filled explant dish onto a shaker plate of a shaker station, operate the shaker station to move the shaker plate in a direction within a plane defined by the shaker plate to infect the plurality of explants with the Agrobacterium solution, and operate the second robotic arm to move an explant from the filled explant dish to a predetermined position on a cultivation media dish in response to a determination that the explant has been infected with the Agrobacterium solution.

19 . The explant preparation apparatus of claim 18 , further comprising a third robotic arm including a claw grip to grasp explant dishes for movement.

20 . The explant preparation apparatus of claim 18 , wherein:
the first robotic arm includes a compressed air source, and the electronic controller is configured to operate the compressed air source to move the claw grip between an open and closed position.

21 . The explant preparation apparatus of claim 18 , wherein the Agrobacterium solution comprises Agrobacterium tumefaciens.

22 . The explant preparation apparatus of claim 18 , wherein the Agrobacterium solution comprises Agrobacterium rhizogenes.

23 . A dish dispensing system, comprising:
a housing, an elongated body secured to the housing and centered about a longitudinal axis, wherein the elongated body is configured to secure a stack of petri dishes along the longitudinal axis, a first pneumatic device positioned in the housing and configured to move a set of petr

Assignees

• Dow Agrosciences Llc

Inventors

• Mccarty Ii Donald L
• Chennareddy Siva
• Cicak Toby
• Gee William E
• Badour David
• Lund John
• Sarria Rodrigo
• Morabito Paul

Classifications

• C12N15/8205 Primary

  Agrobacterium mediated transformation · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M23/10 Primary

  Petri dish (crystallising dishes B01L3/06) · CPC title

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title