Functionalized chromatographic materials and methods of making and using therefor

Patent metadata

Field	Value
Publication number	US-2020147582-A1
Application number	US-202016743857-A
Country	US
Kind code	A1
Filing date	Jan 15, 2020
Priority date	Aug 6, 2010
Publication date	May 14, 2020
Grant date	—

Abstract

Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.

First claim

1 - 73 . (canceled)

74 . A separation system comprising: a chromatographic medium comprising atoms of a transition metal selected from the group consisting of copper, gold and a combination thereof, present as a stationary phase in a pipette or column, wherein the chromatographic medium comprises selenium-containing functional group (MSCM), and wherein the selenium-containing functional group is linked to a support by at least one spacer, and a sample source coupled to the pipette or column comprising a sample to be separated, wherein the sample includes a compound having a functional group.

75 . The chromatographic medium according to claim 74 , on a support selected from the group consisting of silica gel, alumina, polystyrene, agarose, modified polymeric resin, cellulose, magnesium silicate, dextran, and starch.

76 . The chromatographic medium according to claim 74 , configured as an analytical component of a chromatographic separation system selected from: normal phase chromatography, reversed-phase chromatography, liquid chromatography, planar chromatography, column chromatography, flush chromatography, flash chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography, and solid phase extraction chromatography.

77 . The chromatographic medium according to claim 74 , wherein the selenium-containing functional group is linked to the support medium by at least one spacer selected from: (C1-C18)alkyl, (C1-C18)alkoxy, (C1-C18)heteroalkyl, (C6-C10)aryl, (C1-C9)heteroaryl, and (C6-C10)aryl(C1-C6)alkyl.

78 . The chromatographic medium according to claim 74 , wherein the MSCM further comprises a covalently modified silicon atom selected from: a (C1-C18)alcohol, a (C1-C18)nitrile, a (C1-C18)carbonyl, a (C1-C18)ester, and a (C1-C18)alkoxy.

Assignees

• Univ Brown

Inventors

• Huang Yongsong
• Aponte Jose C
• Tarozo Rafael
• Dillon James

Classifications

• B01J20/284

  based on alumina · CPC title

• B01J20/289

  bonded via a spacer · CPC title

• B01J2220/62

  In a cartridge · CPC title

• B01J20/3242

  Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group · CPC title

• B01J20/291

  Gel sorbents · CPC title