Functionalized chromatographic materials and methods of making and using therefor

Patent metadata

Field	Value
Publication number	US-10549255-B2
Application number	US-201313760497-A
Country	US
Kind code	B2
Filing date	Feb 6, 2013
Priority date	Aug 6, 2010
Publication date	Feb 4, 2020
Grant date	Feb 4, 2020

Abstract

Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.

First claim

What is claimed is:

1. A separation system comprising:
a silver thiolate chromatographic medium present as a stationary phase in a pipette or column, wherein the chromatographic medium comprises a sulfur-containing functional group wherein the sulfur is covalently bound to a silver atom, and
wherein the sulfur-containing functional group is linked to a support by at least one spacer selected from: a (C 1 -C 15 )alkyl, a (C 1 -C 15 )alkoxy, a (C 1 -C 15 )heteroalkyl, a (C 6 -C 10 )aryl, a (C 1 -C 9 )heteroaryl, and a (C 6 -C 10 )aryl(C 1 -C 6 )alkyl, and
a sample source coupled to the pipette or column comprising a sample to be separated, wherein the sample includes a compound having a functional group selected from the group consisting of alkenyls, alkynyls, aromatics, and amines.

2. The chromatographic medium according to claim 1 , on a support selected from the group consisting of: silica gel, alumina, polystyrene, agarose, modified polymeric resin, cellulose, magnesium silicate, dextran, and starch.

3. The chromatographic medium according to claim 1 , configured as an analytical component of a chromatographic separation system selected from: normal phase chromatography, reversed-phase chromatography, liquid chromatography, planar chromatography, column chromatography, flush chromatography, flash chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography, and solid phase extraction chromatography.

Assignees

• Univ Brown

Inventors

• Huang Yongsong
• Aponte Jose C
• Tarozo Rafael
• Dillon James

Classifications

• B01J20/291

  Gel sorbents · CPC title

• B01J20/3219

  involving a particular spacer or linking group, e.g. for attaching an active group · CPC title

• B01J2220/62

  In a cartridge · CPC title

• B01J20/3208

  Polymeric carriers, supports or substrates · CPC title

• B01J20/289

  bonded via a spacer · CPC title