Highly efficient aerobic phosphorus-removing bacteria capable of synthesizing nanoparticles by microbial self-assembly using waste water

Patent metadata

Field	Value
Publication number	US-2019071335-A1
Application number	US-201716093639-A
Country	US
Kind code	A1
Filing date	Mar 2, 2017
Priority date	Apr 14, 2016
Publication date	Mar 7, 2019
Grant date	—

Abstract

The present application discloses a class of aerobic efficient-phosphorus-removal bacteria that enable to biologically self-assemble and synthesize nanoparticles while wastewater treatment, including Shewanella sp. CF8-6, Psychrobacter aquimaris X3-1403, and Erythrobacter citreus X3-1411. The strains in the present application have a high adaptability, which may grow, remove nutrients including phosphorus and synthesize nanoparticles within a broad range of pH values, salinity, temperatures, and nutrition concentrations of wastewater. Particularly, the outstanding performance of phosphorous removal at high-salinity has a high significance in wastewater treatment from seawater utilization such as seawater toilet-flushing to solve the fresh water resource deficiency. Self-flocculation and self-assembly are the important properties of the strains to form biofilms and synthesize calcium phosphate nanoparticles at low-concentrations, while decomposing contaminants in the wastewater. The application provides an environmental-friendly nanoparticle synthesis method with low-cost and without chemical additives, which realizes the efficient treatment of wastewater and high value phosphorous resources recovery.

First claim

1 . A class of aerobic efficient-phosphorus-removal bacteria that enable to biologically self-assemble and synthesize nanoparticles while wastewater treatment, include Shewanella sp. CF8-6, Psychrobacter aquimaris X3-1403, and Erythrobacter citreus X3-1411, among which:
the Shewanella sp. CF8-6 was collected in China Center for Type Culture Collection on Mar. 29, 2016 at Luojiashan, Wuchang, Wuhan City, with a collection number of CCTCC M 2016154; the Psychrobacter aquimaris X3-1403 was collected in China Center for Type Culture Collection on Mar. 29, 2016 at Luojiashan, Wuchang, Wuhan City, with a collection number of CCTCC M 2016155; and the Erythrobacter citreus X3-1411 was collected in China Center for Type Culture Collection on Mar. 29, 2016 at Luojiashan, Wuchang, Wuhan City, with a collection number of CCTCC M 2016156.

2 . A microbial agent with an active ingredient selected from at least one bacterium ( Shewanella sp. CF8-6, Psychrobacter aquimaris X3-1403, and Erythrobacter citreus X3-1411) in claim 1 .

3 . The microbial agent in claim 2 may include a solid of liquid carrier.

4 . The microbial agent according to claim 2 , wherein the active ingredient is the cultured living cell, a fermentation broth of the living cell, a filtrate of a cell culture, or a mixture of cell and filtrate.

5 . The microbial agent according to claim 2 , wherein a dosage form of the microbial agent is liquor, suspension concentrate, powder, granules, wettable powder, or water dispersible granules.

6 . (canceled)

7 . (canceled)

8 . (canceled)

9 . (canceled)

10 . (canceled)

11 . A self-assembled biological nanomaterial, which is synthesized by a strain according to claim 1 or Pseudoalteromonas sp. DSBS with a collection number of CCTCC M2013652 in phosphorous-containing wastewater and prepared through self-assembly.

12 . The self-assembled biological nanomaterial in claim 11 , wherein a concentration of phosphorous in the phosphorous-contained wastewater is 0.3 mM˜1.3 mM.

13 . (canceled)

14 . (canceled)

15 . (canceled)

16 . (canceled)

17 . (canceled)

Assignees

• Univ Shandong

Inventors

• Zhou Weizhi
• Jiang Li
• Huang Zhaosong
• Wang Yanru
• Zhao Haixia

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C02F3/341 Primary

  Consortia of bacteria · CPC title

• C02F3/308

  Biological phosphorus removal · CPC title

• C02F2103/08

  Seawater, e.g. for desalination · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title