Microorganisms-immobilized felt-based resin for the treatment of phenolic effluents and a preparation method thereof

Patent metadata

Field	Value
Publication number	US-9758415-B2
Application number	US-201314025353-A
Country	US
Kind code	B2
Filing date	Sep 12, 2013
Priority date	Oct 15, 2012
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

A method of preparing a microorganisms-immobilized felt-based resin includes the following steps: providing a mixture of an acrylate monomer, an initiator, a solvent, and water; adding a felt to the mixture; initiating a polymerization reaction of the mixture to form a felt-based resin; and immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin.

First claim

What is claimed is:

1. A method of preparing a microorganisms-immobilized felt-based resin comprising:
providing a mixture consisting of an acrylate monomer, an initiator, an organic solvent, a felt, and water,
initiating a polymerization reaction of the mixture consisting of the acrylate monomer, the initiator, the organic solvent, the felt, and water to form a felt-based resin, and
immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin,
wherein the acrylate monomer is polymerized on the felt with a uniform distribution to form the felt-based resin in the mixture consisting of the acrylate monomer, the initiator, the organic solvent, the felt, and water;
wherein the organic solvent is selected from the group consisting of ethyl acetate, toluene, N-methyl-2-pyrollidinone, propylene carbonate, ethylene carbonate, butylene carbonate, dimethyl carbonate, diethyl carbonate, 1,2-dimethoxy ethane, 2-methyl tetrahydrofuran, dimethylsulfoxide, 1,3-dioxolane, formamide, dimethylformamide, dioxolane, acetonitrile, nitromethane, methyl formate, methyl acetate, phosphoric acid triester, trimethoxy methane, methyl sulfolane, 1,3-dimethyl-2-imidazolidinone, methyl propionate, and ethyl propionate;
wherein the acrylate monomer is selected from the group consisting of methyl acrylate, ethyl acrylate, propyl acrylate, isopropyl acrylate, n-butyl acrylate, isobutyl acrylate, n-amyl acrylate, isoamyl acrylate, n-ethyl hexyl acrylate, 2-ethyl hexyl acrylate, 2-hydroxy ethyl acrylate, methyl methacrylate, ethyl methacrylate, propyl methacrylate, isopropyl methacrylate, n-butyl methacrylate, isobutyl methacrylate, n-amyl methacrylate, isoamyl methacrylate, n-hexyl methacrylate, n-ethyl hexyl methacrylate, 2-ethyl hexyl methacrylate, hydroxyethyl methacrylate, and hydroxypropyl methacrylate;
wherein the initiator is an organic peroxide initiator or an azo initiator; and
wherein the felt includes fibers with a network structure, and the microorganisms are distributed on the surface and interspace of the fibers.

2. The method of claim 1 , wherein the microorganisms are selected from the group consisting of Pseudomonas putida, Candida albicans, Rhodococcus , and Trichosporon.

3. The method of claim 1 , wherein the initiator is in an amount of 0.01 wt % to 5 wt % based on the weight of the acrylate monomer, the organic solvent is in an amount of 100 wt % to 200 wt % based on the weight of the acrylate monomer, and water is in an amount of 100 wt % to 500 wt % based on the weight of the acrylate monomer.

4. The method of claim 1 , the felt is selected from the group consisting of polypropylene fiber, acrylic fiber, polyacrylonitrile fiber, sponge, aramid fiber, and non-woven fabrics.

5. The method of claim 1 , wherein the microorganisms are treated in an acclimatization process before immobilization.

6. The method of claim 5 , wherein the acclimatization process comprising gradually increasing a phenol concentration and gradually decreasing a glucose concentration.

7. The method of claim 6 , wherein the phenol concentration increases from 0 to 2000 mg/L at a rate of 100 mg/L per day.

8. The method of claim 6 , wherein the glucose concentration decreases from 1000 mg/L at a rate of 100 mg/L per day.

9. A microorganisms-immobilized felt-based resin prepared by the following steps:
providing a mixture consisting of an acrylate monomer, an initiator, an organic solvent, a felt and water,
initiating a polymerization reaction of the mixture consisting of the acrylate monomer, the initiator, the organic solvent, the felt, and water to form a felt-based resin, and
immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin,
wherein the acrylate monomer is polymerized on the felt with a uniform distribution to form the felt-based resin in the mixture consisting of the acrylate monomer, the initiator, the organic solvent, the felt, and water;
wherein the organic solvent is selected from the group consisting of ethyl acetate, toluene, N-methyl-2-pyrollidinone, propylene carbonate, ethylene carbonate, butylene carbonate, dimethyl carbonate, diethyl carbonate, 1,2-dimethoxy ethane, 2-methyl tetrahydrofuran, dimethylsulfoxide, 1,3-dioxolane, formamide, dimethylformamide, dioxolane, acetonitrile, nitromethane, methyl formate, methyl acetate, phosphoric acid triester, trimethoxy methane, methyl sulfolane, 1,3-dimethyl-2-imidazolidinone, methyl propionate, and ethyl propionate;
wherein the initiator is an organic peroxide initiator or an azo initiator;
wherein the acrylate monomer is selected from the group consisting of methyl acrylate, ethyl acrylate, propyl acrylate, isopropyl acrylate, n-butyl acrylate, isobutyl acrylate, n-amyl acrylate, isoamyl acrylate, n-ethyl hexyl acrylate, 2-ethyl hexyl acrylate, 2-hydroxy ethyl acrylate, methyl methacrylate, ethyl methacrylate, propyl methacrylate, isopropyl methacrylate, n-butyl methacrylate, isobutyl methacrylate, n-amyl methacrylate, isoamyl methacrylate, n-hexyl methacrylate, n-ethyl hexyl methacrylate, 2-ethyl hexyl methacrylate, hydroxyethyl methacrylate, and hydroxypropyl methacrylate; and
wherein the felt includes fibers with a network structure, and the microorganisms are distributed on the surface and interspace of the fibers.

10. A method of cleaning a phenolic effluent comprising:
preparing a microorganisms-immobilized felt-based resin prepared in accordance with the method of claim 1 , and
treating the phenolic effluent with the microorganisms-immobilized felt-based resin.

11. The method of claim 10 , wherein the phenolic effluent has a phenol concentration of 1000-5000 mg/L.

Assignees

• Univ Soochow

Inventors

• Lu Jianmei
• Li Najun

Classifications

• C02F2101/345

  Phenols · CPC title

• C02F2103/365

  from petrochemical industry (e.g. refineries) · CPC title

• C02F3/103

  Textile-type packing · CPC title

• C02F3/341

  Consortia of bacteria · CPC title

• C12N11/087

  Acrylic polymers · CPC title