Method for Engineering Three-Dimensional Synthetic Vascular Networks Through Mechanical Micromachining and Mutable Polymer Micromolding
US-2015376595-A1 · Dec 31, 2015 · US
US2017298339A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017298339-A1 |
| Application number | US-201715481600-A |
| Country | US |
| Kind code | A1 |
| Filing date | Apr 7, 2017 |
| Priority date | Oct 7, 2014 |
| Publication date | Oct 19, 2017 |
| Grant date | — |
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Engineered stable multi-organism (or multi-cell type) communities encapsulated in a media that slows or prohibits certain metabolic functions such as cell division, but maintains other metabolic functions.
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What is claimed is: 1 . An artificial biofilm comprising a metabolically active and stable, encapsulated population or community of multiple types of microorganisms or cells. 2 . The artificial biofilm of claim 1 wherein the community is encapsulated in a silica-based gel. 3 . The artificial biofilm of claim 1 wherein the encapsulated population comprises a bacteria, algae, or cyanobacterium. 4 . The artificial biofilm of claim 1 comprising multiple modules wherein each module comprises at least one type of encapsulated microorganism or cell. 5 . The artificial biofilm of claims 4 wherein a first encapsulated microorganism is bacteria. 6 . The artificial biofilm of claim 4 wherein the second encapsulated organism comprises eukaryotic cells. 7 . The artificial biofilm of claim 5 wherein the bacteria is electrochemically active. 8 . The artificial biofilm of claim 7 wherein the bacteria is selected from the group consisting of Shewanella oneidensis, Geobacter sulfurreducens, Shewanella putrefaciens, or Aeromaonas hydrophila. 9 . The artificial biofilm of claim 5 wherein the bacteria is a dissimilatory metal reducing bacteria. 10 . The artificial biofilm of claim 9 wherein the bacteria is Shewanella oneidensis. 11 . The artificial biofilm of claim 4 wherein a first encapsulated microorganism is algae. 12 . The artificial biofilm of claim 5 wherein a second encapsulated microorganism is algae. 13 . The artificial biofilm of claims 5 wherein a second encapsulated microorganism is a cyanobacterium. 14 . The artificial biofilm of claims 5 wherein a second encapsulated microorganism is a heterotroph. 15 . The artificial biofilm of claim 12 wherein the first encapsulated microorganism is a dissimilatory metal reducing bacteria, and a third encapsulated microorganism is a second dissimilatory metal reducing bacteria or an acetate/methanogen syntroph. 16 . A method for producing electricity and reusable water from waste water comprising: exposing the waste water to a microbial fuel cell comprising an electrode comprising artificial biofilm comprising a metabolically active and stable, encapsulated population or community of multiple types of microorganisms or cells.
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