Composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid and their derivatives and method of preparation thereof

Patent metadata

Field	Value
Publication number	US-2018221429-A1
Application number	US-201815944616-A
Country	US
Kind code	A1
Filing date	Apr 3, 2018
Priority date	May 26, 2009
Publication date	Aug 9, 2018
Grant date	—

Abstract

A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent, and a method for use in the preparation of a composition containing caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives. The method includes the steps of pre-concentrating an extract containing the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives and isolating the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives from said extract by liquid chromatography, wherein the elution activity of said caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, with the caffeoylshikimic acids and their derivatives varying depending on the stationary phase and the composition of the mobile phase.

First claim

We claim:

1 . A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent.

2 . The composition as claimed in claim 1 , wherein the composition comprises caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from the vegetation liquor of palm oil milling.

3 . The composition as claimed in claim 1 , wherein the composition further contains at least one of the following: hydroxytyrosol, p-hydroxybenzoic acid, 5-0-caffeoylshikimic acid, 4-0-caffeoylshikimic acid and 3-0-caffeoylshikimic acid.

4 . A method for use in the preparation of a composition containing caffeoylshikimic acids and their derivatives, said method comprising the steps of providing an extract from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent pre-concentrating said extract containing said caffeoylshikimic acids and their derivatives and isolating said caffeoylshikimic acids and their derivatives from said extract by liquid chromatography, wherein the elution activity of said caffeoylshikimic acids and their derivatives results to a plurality of peaks on said liquid chromatography; wherein the elution activity occurs at 40, 44 and 48 minutes within at least one fraction of the extract, thereby producing at least three peaks by liquid chromatography.

5 . A method for use in the preparation of a composition containing caffeoylshikimic acids and their derivatives as claimed in claim 4 , wherein the pre-concentrating step further comprising the steps of loading a column with the extract, removing the impurities, eluting the extract with methanol or ethanol, concentrating in a rotary evaporator, adding an amount of cold ethanol, storing at −20° C., centrifuging said mixture, dissolving the precipitate obtained from centrifuging with an amount of water and concentrating said mixture by rotary evaporator at 50° C.

6 . A method as claimed in claim 5 wherein the amount of cold ethanol is at least 3 volumes of the extract.

7 . A method as claimed in claim 7 5 wherein the amount of water for dissolving the precipitate is approximately 10 ml.

8 . A method as claimed in claim 4 wherein in the isolation step, the following parameters are provided for liquid chromatography; providing a C18 column with 25 length and 10 mm id, using at least one solvent containing an amount of trifluoroacetic acid with water and another solvent containing another amount of trifluoroacetic acid with acetonitrile for mobile phase gradient.

9 . A method as claimed in claim 4 wherein at least one solvent contains 0.1% trifluoroacetic acid and another solvent contains 10/90 0.1% trifluoroacetic acid per acetonitrile.

10 . A method as claimed in claim 4 wherein the percentage of purity of the caffeoylshikimic acids and their derivatives obtained upon isolated is within 90 to 95%.

Assignees

• Malaysian Palm Oil Board

Inventors

• Sambandan T G
• Rha Chokyun
• Sinskey Anthony J
• Sambanthamurthi Ravigadevi
• Tan Yew Ai
• P Manickam Kalyana Sundram
• Wahid Mohd Basri

Classifications

• A61K36/889 Primary

  Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto · CPC title