Plant promoter for transgene expression

Patent metadata

Field	Value
Publication number	US-2018094271-A1
Application number	US-201715698714-A
Country	US
Kind code	A1
Filing date	Sep 8, 2017
Priority date	Oct 3, 2016
Publication date	Apr 5, 2018
Grant date	—

Abstract

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

First claim

What is claimed is:

1 . A nucleic acid vector comprising a promoter operably linked to:
a) a polylinker sequence; b) a non- Panicum virgatum (Pavir.J00490) egg cell heterologous coding sequence; or c) a combination of a) and b); wherein said promoter comprises a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.

2 . The nucleic acid vector of claim 1 , wherein said promoter is 1,290 bp in length.

3 . The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.

4 . The nucleic acid vector of claim 1 , further comprising a sequence encoding a selectable maker.

5 . The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.

6 . The nucleic acid vector of claim 5 , wherein the heterologous coding sequence encodes a selectable marker, an insecticidal resistance protein, a small RNA molecule, a site specific nuclease protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a nutritional quality protein or a DNA binding protein.

7 . The nucleic acid vector of claim 1 , further comprising a 3′ untranslated polynucleotide sequence.

8 . The nucleic acid vector of claim 1 , further comprising a 5′ untranslated polynucleotide sequence.

9 . The nucleic acid vector of claim 1 , further comprising an intron sequence.

10 . The nucleic acid vector of claim 1 , wherein said promoter has embryonic cell preferred expression.

11 . A transgenic plant comprising a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1 operably linked to a heterologous coding sequence.

12 . The transgenic plant of claim 11 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.

13 . The transgenic plant of claim 12 , wherein said plant is Zea mays.

14 . The transgenic plant of claim 11 , wherein the heterologous coding sequence is inserted into the genome of said plant.

15 . The transgenic plant of claim 11 , wherein a promoter comprises a polynucleotide sequence having at least 95% sequence identity with SEQ ID NO:1 and said promoter is operably linked to a heterologous coding sequence.

16 . The transgenic plant of claim 15 , further comprising a 3′ untranslated sequence.

17 . The transgenic plant of claim 15 , wherein said heterologous coding sequence having embryonic cell tissue preferred expression.

18 . The transgenic plant of claim 15 , wherein said promoter is 1,290 bp in length.

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Barone Pierluigi
• Hemingway Daren
• Etchison Emily
• Asberry Andrew
• Pence Heather
• Bowling Andrew J

Classifications

• C12N15/8233 Primary

  Female-specific, e.g. pistil, ovule · CPC title

• C12N15/8234 Primary

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/823 Primary

  Reproductive tissue-specific promoters · CPC title

• C12N15/8209

  Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title