AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use

Patent metadata

Field	Value
Publication number	US-10125372-B2
Application number	US-201615097566-A
Country	US
Kind code	B2
Filing date	Apr 13, 2016
Priority date	Feb 2, 2004
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

First claim

What is claimed:

1. A method of promoting embryogenesis in a microspore and anther cultures, wherein said method comprises introducing into a plant an ODP2 nucleotide sequence that encodes an ODP2 polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO: 2;
(b) the amino acid sequence having at least 85% sequence identity to SEQ ID NO: 2, wherein said polypeptide renders a plant cell embryogenic.

2. The method of claim 1 , wherein said ODP2 nucleotide sequence is under the control of a tissue specific promoter that is active in a microspore.

3. The method of claim 1 , wherein said ODP2 nucleotide sequence is under the control of a promoter that is active during microspore development.

4. The method of claim 3 , wherein said promoter that is active during microspore development is the maize PG47 promoter or the zm-G13 promoter.

5. The method of claim 1 , wherein the ODP2 nucleotide sequence is under the control of an inducible promoter.

6. The method of claim 1 , wherein the ODP2 nucleotide sequence is under the control of a promoter that is an inducible pollen-specific promoter.

Assignees

• Pioneer Hi Bred Int
• Pioneer Hi Bred Int

Inventors

• Gordon-Kamm William J
• Helentjaris Timothy G
• Lowe Keith S
• Shen Bo
• Tarczynski Mitchell C
• Zheng Peizhong

Classifications

• C12N15/8231

  Male-specific, e.g. anther, tapetum, pollen · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C12N15/8287

  for fertility modification, e.g. apomixis · CPC title

• C12N15/8233

  Female-specific, e.g. pistil, ovule · CPC title