Polynucleotides and polypeptides involved in plant fiber development and methods of using same

Patent metadata

Field	Value
Publication number	US-9834781-B2
Application number	US-201514667895-A
Country	US
Kind code	B2
Filing date	Mar 25, 2015
Priority date	Jun 14, 2004
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NO: 121, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 95 or 96, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.

First claim

What is claimed is:

1. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ ID NO: 117 and a heterologous promoter for regulating expression of said nucleic acid sequence in a host cell, wherein the isolated polynucleotide is operably linked to said heterologous promoter, wherein a plant transformed with said construct exhibits an increase in biomass.

2. The nucleic acid construct of claim 1 , wherein said polypeptide is at least 99% identical to the polypeptide set forth by SEQ ID NO: 117.

3. The nucleic acid construct of claim 1 , wherein said polypeptide is set forth by SEQ ID NO: 117.

4. The nucleic acid construct of claim 1 , wherein said polynucleotide is set forth by SEQ ID NO: 12.

5. The nucleic acid construct of claim 1 , wherein said promoter is set forth in SEQ ID NO: 91.

6. The nucleic acid construct of claim 1 , wherein said promoter is a constitutive promoter.

7. The nucleic acid construct of claim 1 , wherein said promoter is selected from the group consisting of an inducible promoter, an endothelial cell promoter, a tissue-specific promoter and a developmental stage-specific promoter.

8. A transgenic plant cell comprising a nucleic acid construct comprising an isolated polynucleotide encoding a polypeptide having an amino acid sequence having at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ. ID NO: 117, wherein a plant transformed with said construct exhibits an increase in biomass, and wherein the isolated polynucleotide is operably linked to a heterologous promoter for regulating expression of said polynucleotide in a plant cell.

9. The transgenic plant cell of claim 8 , wherein said cell forms part of a plant.

10. The transgenic plant cell of claim 8 , wherein the plant is a monocot plant.

11. The transgenic plant cell of claim 8 , wherein the plant is a dicot plant.

12. A transgenic plant comprising the nucleic acid construct of claim 2 .

13. A method of generating a transgenic plant, comprising introducing into a cell of the plant the nucleic acid construct of claim 1 , thereby generating the transgenic plant.

14. A method of improving biomass, yield and/or growth rate of a plant as compared to a non-transformed plant, the method comprising transforming a plant with a nucleic acid construct which comprises an isolated polynucleotide encoding a polypeptide having, an amino acid sequence at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ ID NO: 117, and wherein the isolated polynucleotide is operably linked to a heterologous promoter for regulating expression of said polynucleotide in a plant cell, wherein expression of said polynucleotide improves biomass, yield and/or growth rate of the plant as compared to the nontransformed plant.

15. A transgenic plant comprising the nucleic acid construct of claim 3 .

16. A transgenic plant comprising the nucleic acid construct of claim 4 .

17. The method of claim 14 , wherein said polypeptide is set forth by SEQ ID NO: 117.

18. The method of claim 14 , wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO 12.

Assignees

• Evogene Ltd

Inventors

• Ronen Gil
• Gold Evgenia
• Yelin Rodrigo
• Meissner Rafael
• Karchi Hagai
• Ayal Sharon

Classifications

• C12Q2600/13

  Plant traits · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12N15/8255 Primary

  involving lignin biosynthesis · CPC title