Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
US2018009855A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018009855-A1 |
| Application number | US-201715712172-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 22, 2017 |
| Priority date | Mar 24, 2015 |
| Publication date | Jan 11, 2018 |
| Grant date | — |
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A gene for biosynthesis of core structure of ophiobolin, the gene being the AuOS gene of Aspergillus sp. 094102, deposited with the accession number CCTCC No: M208153, the gene sequence thereof being shown as SEQ ID NO. 1. Also provided is a method of preparation of ophiobolin using the gene.
Opening claim text (preview).
The invention claimed is: 1 . A gene for biosynthesis of ophiobolin, wherein the gene is AuOS gene of Aspergillus sp. 094102, deposited with the accession number CCTCC No: M208153, and a gene sequence thereof is shown in SEQ ID NO. 1. 2 . The gene of claim 1 , wherein the AuOS gene has a cDNA sequence shown in SEQ ID NO. 2. 3 . An AuOS protein being encoded by the gene of claim 1 , the protein having an amino acid sequence shown in SEQ ID NO. 3. 4 . A method of preparation of ophiobolin, the method comprising applying a gene comprising a sequence shown in SEQ ID NO. 1 or SEQ ID NO. 2, or an AuOS protein having an amino acid sequence shown in SEQ ID NO. 3.
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