Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicyclic compound and uses thereof

Patent metadata

Field	Value
Publication number	US-9458483-B2
Application number	US-201514713751-A
Country	US
Kind code	B2
Filing date	May 15, 2015
Priority date	Aug 12, 2010
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a bicyclic compound. The present invention also relates to methods of using the compositions.

First claim

What is claimed is:

1. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of a GH61 polypeptide having cellulolytic enhancing activity and a bicyclic compound;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation,
wherein the bicyclic compound is of formula (I) or (II):

GH
⁢

⁢
61
⁢

⁢
effect

=

%
⁢

⁢

conversion

(

+
GH

⁢

⁢
61

)

%
⁢

⁢

conversion

(

no
⁢

⁢
GH
⁢

⁢
61

)

.

wherein the bond indicated with a dashed line can be single or double;
R 1 and R 2 are independently hydrogen, —C(O)R 5 , —C(O)OR 6 , —C(O)NHR 7 , or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
R 3 , R 4a , R 4b , and R 4c are independently hydrogen, halogen, —OH, —OR 8 , —CN, —NO 2 , —N(R 9 )(R 10 ), —C(O)R 5 , —C(O)OR 6 , —C(O)NHR 7 , —OC(O)R 11 , —NHC(O) R 12 , —OC(O)OR 13 , —NHC(O)OR 14 , —OC(O)NHR 15 , —NHC(O)NHR 16 , —SO 2 R 17 , —SO 2 N(R 18 )(R 19 ), —SR 20 , or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and wherein two of R 4a , R 4b , and R 4c may together form an optionally substituted fused ring;
R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 18 , R 19 , and R 20 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
R 17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
or a salt or solvate thereof;
wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the bicyclic compound enhances hydrolysis of a cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:

GH
⁢

⁢
61
⁢

⁢
effect

=

%
⁢

⁢

conversion

(

+

⁢
GH

⁢

⁢
61

)

%
⁢

⁢

conversion

(

no
⁢

⁢
GH
⁢

⁢
61

)

.

2. The method of claim 1 , wherein the bicyclic compound is of the formula (I-A) or (II-A):

or a salt or solvate thereof.

3. The method of claim 1 , wherein the bicyclic compound is of the formula (I-B) or (II-B):

or a salt or solvate thereof.

4. The method of claim 1 , wherein the bicyclic compound is of the formula (I-C), (I-D), (I-E), or (I-F):

or a salt or solvate thereof.

5. The method of claim 1 , wherein the bicyclic compound

Assignees

• Novozymes Inc

Inventors

• Quinlan Jason
• Xu Feng
• Sweeney Matthew

Classifications

• C12N9/96

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title