Cell culture container

Patent metadata

Field	Value
Publication number	US-2016355773-A1
Application number	US-201615240284-A
Country	US
Kind code	A1
Filing date	Aug 18, 2016
Priority date	Feb 20, 2014
Publication date	Dec 8, 2016
Grant date	—

Abstract

A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

First claim

What is claimed is:

1 . A cell culture container, comprising:
a container main body; and a flat plate attached to one surface of the container main body, wherein the container main body includes an inflow port through which a liquid flows into the container main body, a passage through which the liquid flowing into the container main body from the inflow port passes, and an outflow port through which the liquid passing through the passage flows out from the container main body, and a plurality of cell-seeding areas, in which cells passing through the passage are seeded, is provided on a bottom surface of the passage and is arranged side by side along the passage, wherein a diameter of the passage is from 2 mm to 4 mm.

2 . The container of claim 1 , wherein a ratio of width and length of the passage is set such that the width:the length is equal to 3:60 to 1800.

3 . The container of claim 1 , wherein the flat plate is fixedly supported by a tip region of a wall that defines the passage.

4 . The container of claim 1 , wherein the cell-seeding areas are provided at regular intervals along the passage.

5 . The container of claim 1 , wherein the passage includes a serpentine portion.

6 . The container of claim 1 , wherein the passage includes a branching section in which the passage is branched into a plurality of passage components and a merging section in which the passage components are merged.

7 . The container of claim 1 , wherein cavities are formed on the bottom surface of the passage in a concentric relationship with the cell-seeding areas.

8 . The container of claim 7 , wherein the cavities have a pyramidal shape or a conical shape.

9 . The container of claim 7 , wherein each of the cavities includes a flat bottom portion.

10 . The container of claim 7 , wherein an extracellular matrix (ECM) is coated on a portion of the flat plate which faces the cavities.

11 . The container of claim 9 , wherein an extracellular matrix (ECM) is coated inside the cavities and is not coated outside the cavities.

12 . The container of claim 1 , wherein the bottom surface of the passage is flat and an extracellular matrix (ECM) is coated inside the cell-seeding areas and is not coated outside the cell-seeding areas.

13 . The container of claim 1 , wherein an outer surface roughness of the cell-seeding areas is larger than an inner surface roughness of the cell-seeding areas.

14 . The container of claim 1 , wherein the bottom surface of the passage and the flat plate have optical transparency.

15 . The container of claim 1 , wherein the flat plate has gas permeability.

Assignees

• Tokyo Electron Ltd

Inventors

• Kurakazu Tomoaki
• Oshima Yasuhiro
• Kimura Yoshio
• Kagawa Kenichi
• Gomi Shinichi
• Ozaki Shigenori

Classifications

• C12M23/04

  Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence) · CPC title

• C12M25/00

  Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings · CPC title

• C12M29/10

  Perfusion · CPC title

• C12M23/24

  Gas permeable parts · CPC title

• C12M25/06

  Plates; Walls; Drawers; Multilayer plates · CPC title