Agricultural microbial inoculant compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-2016355445-A1
Application number	US-201615170813-A
Country	US
Kind code	A1
Filing date	Jun 1, 2016
Priority date	Jun 2, 2015
Publication date	Dec 8, 2016
Grant date	—

Abstract

The present disclosure provides novel agricultural microbial inoculant compositions for uses in promoting plant growth, plant productivity and/or soil quality. The novel microbial inoculant compositions comprise one or more microbial species, one or more urease inhibitors and/or one or more nitrification inhibitors. The present disclosure also provides fertilizer compositions comprising said microbial inoculant compositions.

First claim

What is claimed is:

1 . An agricultural microbial inoculant composition comprising:
i. at least one agriculturally beneficial Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilis, and any combination thereof; and ii. at least one organic solvent selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and any combination thereof.

2 . The agricultural microbial inoculant composition of claim 1 , wherein the agriculturally beneficial Bacillus species is selected from the group consisting of Bacillus licheniformis, Bacillus thuringiensis, and any combination thereof.

3 . The agricultural microbial inoculant composition of claim 1 , wherein the agriculturally beneficial Bacillus species is selected from the group of strains consisting of Bacillus amyloliquefaciens (ATCC 23842), Bacillus licheniformis (ATCC 14580), Bacillus licheniformis (B-642), Bacillus licheniformis (B-14368), Bacillus thuringiensis (ATCC 10792), Bacillus thuringiensis (HD-17), Bacillus thuringiensis (HD-1), Bacillus pumilis (NRS-272), and any combination thereof.

4 . The agricultural microbial inoculant composition of claim 1 , further comprising a urease inhibitor selected from the group consisting of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene, thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and any combination thereof.

5 . The agricultural microbial inoculant composition of claim 4 , wherein the urease inhibitor is N-(n-butyl)thiophosphoric triamide (NBPT).

6 . The agricultural microbial inoculant composition of claim 1 , further comprising a nitrification inhibitor selected from the group consisting of 2-chloro-6-trichloromethylpyridine, 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazol, dicyandiamide, 2-amino-4-chloro-6-methyl-pyrimidine, 1,3-benzothiazole-2-thiol, 4-amino-N-1,3-thiazol-2-ylbenzene sulfonamide, thiourea, guanidine, 3,4-dimethylpyrazole phosphate, 2,4-diamino-6-trichloromethyl-5-triazine, poly etherionophores, 4-amino-1,2,4-triazole, 3-mercapto-1,2,4-triazole, potassium azide, carbon bisulfide, sodium trithiocarbonate, ammonium dithiocarbamate, 2,3,-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate, N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-alanine methyl ester, ammonium thiosulfate, 1-hydroxypyrazole, 3-methylpyrazole-1-carboxamide, 3-methylpyrazole, 3,5-dimethylpyrazole, 1,2,4-triazole, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7), and any combination thereof.

7 . The agricultural microbial inoculant composition of claim 6 , wherein the nitrification inhibitor is dicyandiamide, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7) or a combination thereof.

8 . The agricultural microbial inoculant composition of claim 4 , wherein the weight percentage of the urease inhibitor is in the range of 0.02-80% based on the total weight of the agricultural microbial inoculant composition.

9 . The agricultural microbial inoculant composition of claim 8 , wherein the weight percentage of the urease inhibitor is in the range of 10-60%.

10 . The agricultural microbial inoculant composition of claim 1 , wherein the agriculturally beneficial Bacillus species has at least 2 hours of viability from the time when said agriculturally beneficial Bacillus species contacts said organic solvent.

11 . The agricultural microbial inoculant composition of claim 1 , wherein the agriculturally beneficial Bacillus species concentration is in the range of 1.0×10 2 1.0×10 12 spores/mL.

12 . The agricultural microbial inoculant composition of claim 1 , further comprising a dye.

13 . The agricultural microbial inoculant composition of claim 4 , comprising propylene glycol and N-methyl-2-pyrrolidone.

14 . The agricultural microbial inoculant composition of claim 13 , wherein the weight percentage of propylene glycol is in the range of 40-70% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of N-methyl-2-pyrrolidone is in the range of 15-40% based on the total weight of the agricultural microbial inoculant composition, and the weight percentage of NBPT is in the range of 10-30% based on the total weight of the microbial inoculant composition.

15 . The agricultural microbial inoculant composition of claim 4 comprising propylene glycol, N-methyl-2-pyrrolidone, and triethylene glycol monobutyl ether.

16 . The agricultural microbial inoculant composition of claim 15 , wherein the weight percentage of propylene glycol is in the range of 10-30% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of N-methyl-2-pyrrolidone is in the range of 30-60% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of triethylene glycol monobutyl ether is in the range of 1-5% based on the total weight of the agricultural microbial inoculant composition, and the weight percentage of NBPT is in the range of 15-40% based on the total weight of the agricultural microbial inoculant composition.

17 . An agricultural microbial inoculant kit comprising:
Part A, wherein Part A comprises a urease inhibitor and at least one organic solvent, wherein said urease inhibitor is selected from the group consisted of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene,thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and any combination thereof; and wherein said organic solvent is selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and any combination thereof; and Part B, wherein Part B comprises at least one Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilis, and any combination thereof. wherein each Part A and Part B is contained in a separate container.

18 . The agricultural microbial inoculant kit of claim 17 , wherein Part B comprises at least one Bacillus species selected from the group consisting of Bacillus licheniformis, Bacillus thuringiensis, and any combination thereof.

19 . The agricultural microbial inoculant kit of claim 17 , Part B comprises at least one agriculturally beneficial Bacillus strain selected from the group consisting of Bacillus amyloliquefaciens (ATCC 23842), Bacillus licheniformis (ATCC 14580), Bacillus licheniformis (B-642), Bacillus licheniformis (B-14368), Bacillus thuringiensis (ATCC 10792), Bacillus thuringiensis (HD-17), Bacillus thuringiensis (HD-1), Bacillus pumilis (NRS-272), and any combination thereof.

Assignees

• Koch Agronomic Services Llc

Inventors

• Bobeck Drew R
• Pearce Cedric J

Classifications

• C05G3/60

  Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants · CPC title

• C05G3/90

  for affecting the nitrification of ammonium compounds or urea in the soil · CPC title

• C05F11/08 Primary

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• A01N25/04

  Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) · CPC title

• C05G3/00

  Mixtures of one or more fertilisers with additives not having a specially fertilising activity · CPC title