Microbial strains, compositions, and methods for increasing available phosphate for plants

Patent metadata

Field	Value
Publication number	US-9365464-B2
Application number	US-201314080546-A
Country	US
Kind code	B2
Filing date	Nov 14, 2013
Priority date	Nov 16, 2012
Publication date	Jun 14, 2016
Grant date	Jun 14, 2016

Abstract

The present invention provides novel phosphate-solubilizing fungal strains, compositions comprising novel phosphate-solubilizing fungal strains, and methods of using novel phosphate-solubilizing fungal strains to increase the availability of phosphate for plant uptake in soil. In some embodiments, one or more of the novel phosphate-solubilizing fungal strains is coated onto a seed.

First claim

The invention claimed is:

1. A method of increasing the availability of phosphorus for plant uptake from a soil, said method comprising introducing into the soil an inoculum that comprises at least one of the Penicillium bilaiae strains listed below:
the Penicillium bilaiae strain having the deposit accession number NRRL B-50777;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50778;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50779;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50780;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50781;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50782;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50783;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50784;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50785;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50786;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50787;
the Penicillium bilaiae strain having the deposit accession number NRRL B-50788.

2. The method of claim 1 , wherein said inoculum is introduced into the soil in an amount ranging from 1×10 1 to 1×10 8 colony-forming units per hectare.

3. The method of claim 1 , wherein said inoculum is introduced into the soil in an amount ranging from 1×10 6 to 1×10 12 colony-forming units per hectare.

4. The method of claim 1 , wherein said inoculum is introduced into the soil as a seed coating.

5. The method of claim 4 , wherein said seed coating comprises 1×10 1 to 1×10 8 colony-forming units per seed.

6. The method of claim 4 , wherein said seed coating comprises 1×10 2 to 1×10 6 colony-forming units per seed.

7. The method of claim 4 , wherein said inoculum is coated on leguminous plant seeds.

8. The method of claim 7 , wherein said leguminous plant seeds are soybean seeds.

9. The method of claim 4 , wherein said inoculum is coated on non-leguminous plant seeds.

10. The method of claim 9 , wherein said non-leguminous plant seeds are corn seeds.

11. The method of claim 1 , wherein said inoculum further comprises a phosphorus source.

12. The method of claim 1 , wherein said inoculum further comprises one or more plant signal molecules.

13. The method of claim 1 , wherein said inoculum further comprises a lipo-chitooligosaccharide (LCO).

14. The method of claim 13 , wherein said LCO is obtained from a species of Rhizobia selected from the group consisting of Rhizobium spp., Bradyrhizobium spp., Sinorhizobium spp., Mesorhizobium spp., and Azorhizobium spp.

15. The method of claim 1 , wherein said inoculum further comprises a chitinous compound.

16. The method of claim 1 , wherein said inoculum further comprises a chito-oligomer (CO).

17. The method of claim 1 , wherein said inoculum further comprises a flavonoid.

18. The method of claim 17 , wherein said flavonoid is luteolin, apigenin, tangeritin, quercetin, kaempferol, myricetin, fisetin, isorhamnetin, pachypodol, rhamnazin, hesperetin, naringenin, eriodictyol, homoeriodictyol, taxifolin, dihydroquercetin, dihydrokaempferol, genistein, daidzein, glycitein, catechin, gallocatechin, catechin 3-gallate, gallocatechin 3-gallate, epicatechin, epigallocatechin, epicatechin 3-gallate, epigallocatechin 3-gallate, cyanidin, delphinidin, malvidin, pelargonidin, peonidin or petunidin.

19. The method of claim 1 , wherein said inoculum further comprises jasmonic acid, linoleic acid, linolenic acid and/or a karrikin.

20. The method of claim 1 , further comprising adding a source of phosphorus to the soil.

Assignees

• Novozymes Bioag As

Inventors

• Greenshields David
• Steckler Shelagh
• Priest Kari
• Caldwell Caressa
• Frodyma Michael

Classifications

• C05G3/60

  Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants · CPC title

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C12N1/14 Primary

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• A01N65/04

  Pteridophyta [fern allies]; Filicophyta [ferns] · CPC title

• C05F11/08 Primary

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title