Method of reducing nitrous oxide emissions from a plant growth substrate

Patent metadata

Field	Value
Publication number	US-9266786-B2
Application number	US-201313843526-A
Country	US
Kind code	B2
Filing date	Mar 15, 2013
Priority date	Oct 17, 2011
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

A method of modulating and in particular, reducing nitrous oxide emission from a substrate for growing on or more plants by applying a microbial based soil additive and a fertilizer blend to the substrate.

First claim

What is claimed is:

1. A method for reducing nitrous oxide emission from a soil composition comprising:
a) applying a fertilizer composition to the soil composition, wherein the fertilizer composition comprises calcium ammonium nitrate, urea ammonium nitrate or urea;
b) applying a Trichoderma -free microbial based soil additive to the soil composition in an amount effective to reduce nitrous oxide emission;
c) measuring the amount of nitrous oxide in the soil composition—fertilizer composition-soil additive combination; and
d) determining that said nitrous oxide emission has been reduced when compared to a control soil-fertilizer composition that has not been treated with the Trichoderma -free microbial based soil additive.

2. The method according to Claim 1 wherein said soil composition is agricultural soil or non-sterile soil in pots.

3. The method according to claim 1 , wherein said soil composition is soil, vermiculite, pearlite, gravel, clay, sand, peat moss, ground up wood, or pine bark.

4. The method according to claim 1 , wherein said microbial based soil additive comprises a mixture of spore-forming plant-growth promoting rhizobacteria comprising an isolate of Bacillus pumilus , an isolate of Bacillus subtilis , an isolate of Bacillus safenis , and an isolate of Lysinibacillus xylanilyticus.

5. The method according to claim 1 wherein said microbial based soil additive comprises Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium and Rhodococcus rhodochrous and has a pH between about 8.0 to about 8.5.

6. The method according to claim 1 , wherein said method further comprises adjusting the moisture level of the soil composition.

7. The method according to claim 1 , wherein the ratio of microbial based liquid soil additive to fertilizer is about 1.5 L (1.5 kg) to about 7.5 L (7.5 kg) per metric ton of granular fertilizer.

8. The method according to claim 1 , wherein the microbial based soil additive and the fertilizer composition are in liquid form, and the ratio of microbial based liquid soil additive to liquid fertilizer composition is from about 1:1 to about 1:50 microbial based additive:undiluted liquid fertilizer composition.

9. The method according to claim 1 , wherein the microbial based soil additive comprises between about 1×10 3 to about 5 ×10 6 colony forming units per milliliter.

10. The method according to claim 1 , wherein the fertilizer is applied at a rate sufficient to provide adequate nitrogen nutrition for growing one or more plants with a fertilizer concentration level of between about 50 mg of fertilizer per kg of soil composition to about 100 mg of fertilizer per kg of soil composition.

Assignees

• Us Agriculture
• Univ Auburn
• Tenfold Technologies Llc
• Tenfold Technologies Llc

Inventors

• Kloepper Joseph W
• Calvo Velez Pamela
• Torbert Iii Henry Allen
• Watts Dexter B
• Ames Robert Norman

Classifications

• C05G3/00 Primary

  Mixtures of one or more fertilisers with additives not having a specially fertilising activity · CPC title

• C05F11/08

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C05C5/00

  Fertilisers containing other nitrates · CPC title

• A01G31/001

  Human Necessities · mapped topic

• Y02P60/216

  Cross-Sectional Technologies · mapped topic