Probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof
US-2024376553-A1 · Nov 14, 2024 · US
Universal probe assay methods
US2016348149A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016348149-A1 |
| Application number | US-201615144529-A |
| Country | US |
| Kind code | A1 |
| Filing date | May 2, 2016 |
| Priority date | Oct 22, 2010 |
| Publication date | Dec 1, 2016 |
| Grant date | — |
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Abstract
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Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
First claim
Opening claim text (preview).
1 . A method for specific detection of a microRNA, comprising: (a) combining (i) a sample comprising the microRNA, (ii) an artificial DNA template comprising a first sequence that is complementary to the microRNA and a second sequence that is 3′ to the first sequence, (iii) reagents for DNA-dependent extension of an RNA primer including a polymerase; thereby producing an extension combination; (b) exposing the extension combination to conditions under which the microRNA anneals to the DNA template and the microRNA is extended by the polymerase thereby producing a chimeric polynucleotide comprising a 5′ microRNA portion and a 3′ DNA portion complementary to the second sequence; and (c) detecting the production of the chimeric polynucleotide.
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Classifications
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title
- C12Q1/682Primary
Signal amplification · CPC title
miRNA, siRNA or ncRNA · CPC title
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Frequently asked questions
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- What does patent US2016348149A1 cover?
- Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to…
- Who is the assignee on this patent?
- Fluidigm Corp
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/682. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Dec 01 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).