Device and method for mixing magnetic bead reagent and sample analysis apparatus

Patent metadata

Field	Value
Publication number	US-12478935-B2
Application number	US-202117506487-A
Country	US
Kind code	B2
Filing date	Oct 20, 2021
Priority date	Apr 22, 2019
Publication date	Nov 25, 2025
Grant date	Nov 25, 2025

Abstract

A mixing device for a magnetic bead reagent includes a magnetic member, a container storage mechanism, and a drive mechanism. The container storage mechanism includes a mounting part for mounting a magnetic bead liquid container storing a magnetic bead reagent, and the mounting part corresponds to the magnetic member, enabling the container on the mounting part to be located within the magnetic field. The drive mechanism has a drive structure capable of driving at least one of the magnetic member, the mounting part, and the container to move, and thus generating a relative movement between the container and the magnetic field. The direction of the magnetic force acting on the magnetic beads is changed by the relative movement between the magnetic bead reagent and the magnetic field, such that the magnetic beads flow along different directions under the magnetic force, thereby increasing mixing efficiency of magnetic bead reagent.

First claim

What is claimed is:

1 . A mixing device for a magnetic bead reagent, comprising:
at least one magnetic member configured to generate a magnetic field; a reagent container storage mechanism having at least two mounting parts, each of the mounting parts being configured to mount a magnetic bead liquid container which stores a magnetic bead reagent to be mixed, the magnetic bead reagent comprising magnetic beads, and the at least two mounting parts being arranged corresponding to the magnetic member, such that the magnetic bead liquid container located on each of the mounting parts is capable of being in the magnetic field, wherein a south pole or a north pole of the magnetic member is oriented toward a side wall of a cavity of the magnetic bead liquid container on each of the mounting parts, wherein the at least two mounting parts are arranged on a periphery of the at least one magnetic member along an arc or a circle, such that the magnetic bead liquid containers located on the at least two mounting parts are capable of being arranged around the at least one magnetic member; and a driving mechanism having a driving structure, wherein the driving structure is capable of driving the magnetic bead liquid container to rotate around a central axis of the magnetic bead liquid container; and wherein the magnetic beads deposited at a bottom of the magnetic bead liquid container are dispersed, so as to mix the magnetic beads in the magnetic bead reagent under the rotation of the magnetic bead liquid container, wherein the driving structure comprises a first driving assembly, and the first driving assembly is capable of driving the magnetic bead liquid container to alternately rotate in forward and reverse directions.

2 . The mixing device of claim 1 , wherein each of the mounting parts has a structure capable of defining an arrangement in which the magnetic bead liquid container extends longitudinally along a first axis of a respective one of the mounting parts, a south pole or a north pole of the magnetic member is arranged in a direction at a non-zero angle with respect to the first axis of the respective one of the mounting parts, and the first axis of the respective one of the mounting parts coincides with the central axis of the magnetic bead liquid container mounted on the respective one of the mounting parts.

3 . The mixing device of claim 2 , wherein the south pole or the north pole of the magnetic member is oriented perpendicularly to the first axis of the respective one of the mounting parts.

4 . The mixing device of claim 1 , wherein the reagent container storage mechanism comprises an annular base, the mounting parts are arranged on the annular base along an arc or a circle, and the magnetic member is located at a central hole of the annular base.

5 . The mixing device of claim 4 , wherein the first driving assembly comprises a first gear disk, a second gear disk which is concentric with and fixedly connected to the annular base, and an electric motor for driving the second gear disk to rotate, wherein the first gear disk is fixedly arranged and located at the central hole of the annular base, and gear teeth of the first gear disk extend into mounting areas of the mounting parts, where the magnetic bead liquid containers are mounted, such that the gear teeth of the first gear disk are capable of meshing with gear teeth on the magnetic bead liquid containers.

6 . The mixing device of claim 5 , wherein the magnetic member is fixedly mounted on the first gear disk.

7 . The mixing device of claim 6 , wherein the at least one magnetic member comprises at least two magnetic members, which are arranged on the first gear disk along an arc or a circle.

8 . The mixing device of claim 7 , wherein the number of the magnetic members is the same as the number of the mounting parts, and each magnetic member corresponds to one mounting part, such that the magnetic bead liquid container located on each mounting part is capable of being located in the magnetic field of the corresponding magnetic member.

9 . The mixing device of claim 5 , wherein the first gear disk is configured to be stationary.

10 . The mixing device of claim 1 , wherein the first driving assembly comprises a first gear disk and an electric motor for driving the first gear disk to rotate, the mounting parts are arranged around the first gear disk along an arc or a circle, and gear teeth of the first gear disk extend into mounting areas of the mounting parts, where the magnetic bead liquid containers are mounted, such that the gear teeth of the first gear disk are capable of meshing with gear teeth on the magnetic bead liquid containers.

11 . The mixing device of claim 1 , wherein the driving structure further comprises a second driving assembly having an output end, and the output end is in transmission connection to the reagent container storage mechanism to drive the at least two mounting parts to rotate around the magnetic member.

12 . The mixing device of claim 1 , wherein each of the mounting parts is provided with at least one of a limiting bulge and a limiting recess for mounting the magnetic bead liquid container.

13 . The mixing device of claim 1 , wherein the south pole or the north pole of the magnetic member is oriented perpendicularly to the side wall of the cavity of the magnetic bead liquid container.

14 . The mixing device of claim 1 , wherein the mixing device further comprises an outer cover configured to cover the top of the mixing device, and the outer cover is provided with an access for inputting or removing the magnetic bead liquid container into or from the mixing device.

15 . A mixing device for a magnetic bead reagent, comprising:
a reagent container storage mechanism having at least two mounting parts, each of the mounting parts being configured to mount a magnetic bead liquid container which stores a magnetic bead reagent to be mixed, wherein the magnetic bead reagent comprises magnetic beads; a magnetic member configured to generate a magnetic field, the magnetic field acting on the magnetic bead reagent in the magnetic bead liquid container, and a south pole or a north pole of the magnetic member being oriented toward a side wall of a cavity of the magnetic bead liquid container on each of the mounting parts, such that the magnetic beads deposited at a bottom of the magnetic bead liquid container are dispersed, so as to mix the magnetic beads in the magnetic bead reagent under rotation of the magnetic bead liquid container; and a driving mechanism comprising a first driving assembly, wherein the first driving assembly is capable of driving the magnetic bead liquid container to alternately rotate in forward and reverse directions, wherein the at least two mounting parts are arranged on a periphery of the magnetic member along an arc or a circle, such that the magnetic bead liquid containers located on the at least two mounting parts are capable of being arranged around the magnetic member.

16 . The mixing device of claim 15 , wherein a south pole or a north pole of the magnetic member is oriented perpendicularly to the side wall of the cavity of the magnetic bead liquid container on each of the mounting parts.

17 . The mixing device of claim 15 , wherein the south pole or the north pole of the magnetic member is oriented perpendicularly to the central axis of the magnetic bead liquid container.

18 . A mixing device for a magnetic bead reagent, comprising:
at least one magnetic member configured to generate a magnetic field, a reagent container stora

Assignees

• Shenzhen Mindray Biomedical Electronics Co Ltd

Inventors

• Xu Wenbo
• Weng Yanwen
• Wang Jun

Classifications

• B01F31/10

  with a mixing receptacle rotating alternately in opposite directions · CPC title

• B01F33/451

  wherein the mixture is directly exposed to an electromagnetic field without use of a stirrer, e.g. for material comprising ferromagnetic particles or for molten metal · CPC title

• B01F29/80

  rotating about a substantially vertical axis · CPC title

• B01F33/813

  mixing simultaneously in two or more mixing receptacles · CPC title

• G01N35/02

  using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations {(G01N35/0098 and G01N35/0099 take precedence)} · CPC title