Cell scaffold comprising an electronic circuit

Patent metadata

Field	Value
Publication number	US-12441973-B2
Application number	US-202017612187-A
Country	US
Kind code	B2
Filing date	Jun 23, 2020
Priority date	Jun 24, 2019
Publication date	Oct 14, 2025
Grant date	Oct 14, 2025

Abstract

The present invention generally relates to nanoscale wires and nanoelectronics, which in some aspects may be embedded in biological structures, such as tissues, organoids, organs, organisms, and the like. For example, one aspect is generally directed to stretchable cell scaffolds, which may be connected to electrical circuits. In some cases, a biological structure may form around the scaffold and deform the cell scaffold as it forms, thereby causing the cell scaffold to become embedded within the biological structure. The scaffold may be connectable in certain embodiments to an external device, e.g., to determine a property of the cell scaffold (e.g., an electrical property), and/or to apply a stimulus (e.g., an electrical stimulus) to the biological structure. Other aspects of the invention are generally directed to methods of making or using such cell scaffolds, kits including such cell scaffolds, biological structures containing such cell scaffolds, or the like.

First claim

What is claimed is:

1. An article, comprising:
a biological structure at least partially embedding a stretchable cell scaffold therein, the cell scaffold defining at least a portion of an electrical circuit,
wherein the biological structure is an embryo or an organoid.

2. The article of claim 1 , wherein the cell scaffold is stretchable by at least 10% in a linear direction.

3. The article of claim 1 , wherein the cell scaffold is foldable by at least 90°.

4. The article of claim 1 , wherein the stretchable cell scaffold has a first filling ratio of less than 40% in the absence of the biological structure.

5. The article of claim 4 , wherein the cell scaffold has a second filling ratio of less than 10% when embedded within the biological structure.

6. The article of claim 1 , wherein the cell scaffold contains at least 30 vol % of the biological structure.

7. The article of claim 1 , wherein at least 30 vol % of the biological structure is no more than 10 micrometers from the cell scaffold embedded therein.

8. The article of claim 1 , wherein the biological structure completely embeds the cell scaffold.

9. The article of claim 1 , wherein at least a portion of the electrical circuit of the cell scaffold is electrically connected to an electrical device external of the biological structure.

10. The article of claim 1 , wherein the cell scaffold comprises a mesh comprising a plurality of nodes, at least some of which nodes are connected by interconnects to form the mesh.

11. The article of claim 10 , wherein the mesh is a rectangular mesh or an irregular mesh.

12. The article of claim 10 , wherein at least some of the interconnects are serpentine and/or stretchable.

13. The article of claim 12 , wherein the interconnects comprise electronically conductive pathways.

14. The article of claim 10 , wherein at least some of the interconnects comprise at least one inflection point.

15. The article of claim 10 , wherein at least some of the interconnects have a maximum cross-sectional dimension of less than 50 micrometers.

16. The article of claim 1 , wherein the cell scaffold comprises a photoresist.

17. The article of claim 16 , wherein the photoresist comprises SU-8, a polymer formed by photo-curing a fluorinated monomer including cross-linkable function groups using a photoinitiator, and/or a hydrogel.

18. The article of claim 16 , wherein the photoresist comprises perfluoropolyether dimethacrylate (PFPE-DMA).

19. The article of claim 1 , wherein the cell scaffold comprises metal pathways.

20. The article of claim 19 , wherein a least some of the metal pathways comprise gold or chromium.

21. The article of claim 19 , wherein a least some of the metal pathways are flexible.

22. The article of claim 1 , wherein the cell scaffold comprises a onductive polymer.

23. The article of claim 1 , wherein the cell scaffold comprises a nanoscale wire.

24. The article of claim 23 , wherein the nanoscale wire is responsive to an electrical property external to the nanoscale wire.

25. An article, comprising:
a cell scaffold defining at least a portion of an electrical circuit, wherein the cell scaffold is stretchable by at least 20% in a linear direction under a force of no more than 1 micronewton,
wherein the biological structure is an embryo or an organoid.

26. A method, comprising:
exposing cells to a stretchable cell scaffold defining at least a portion of an electrical circuit; and
growing the cells to form a biological structure at least partially embedding the cell scaffold therein,
wherein the biological structure is an embryo or an organoid.

Assignees

• Harvard College

Inventors

• Liu Jia
• Nan Kewang
• Li Qiang

Classifications

• C12M35/02

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M35/04

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M25/14 Primary

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title