Scaffolds comprising nanoelectronic components for cells, tissues, and other applications

Patent metadata

Field	Value
Publication number	US-9457128-B2
Application number	US-201314018082-A
Country	US
Kind code	B2
Filing date	Sep 4, 2013
Priority date	Sep 7, 2012
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

The present invention generally relates to nanoscale wires and tissue engineering. In various embodiments, cell scaffolds for growing cells or tissues can be formed that include nanoscale wires that can be connected to electronic circuits extending externally of the cell scaffold. The nanoscale wires may form an integral part of cells or tissues grown from the cell scaffold, and can even be determined or controlled, e.g., using various electronic circuits. This approach allows for the creation of fundamentally new types of functionalized cells and tissues, due to the high degree of electronic control offered by the nanoscale wires and electronic circuits. Accordingly, such cell scaffolds can be used to grow cells or tissues which can be determined and/or controlled at very high resolutions, due to the presence of the nanoscale wires, and such cell scaffolds will find use in a wide variety of novel applications, including applications in tissue engineering, prosthetics, pacemakers, implants, or the like.

First claim

What is claimed is:

1. An article, comprising:
a cell scaffold comprising semiconductor nanoscale wires and one or more polymeric constructs, wherein at least some of the nanoscale wires form a portion of an electrical circuit that extends externally of the cell scaffold and wherein at least one of the one or more polymeric constructs comprises a photoresist.

2. The article of claim 1 , wherein the cell scaffold comprises a biocompatible polymer.

3. The article of 1 , wherein the cell scaffold comprises a biodegradable polymer.

4. The article of claim 1 , wherein the cell scaffold comprises an extracellular matrix protein.

5. The article of claim 1 , wherein the cell scaffold has an open porosity of at least about 50%.

6. The article of claim 1 , wherein the cell scaffold has an areal mass density of less than about 60 micrograms/cm 2 .

7. The article of claim 1 , wherein at least some of nanoscale wires form part of a field effect transistor within the electrical circuit.

8. The article of claim 1 , wherein at least one of the nanoscale wires is pH-sensitive.

9. The article of claim 1 , wherein at least one of the nanoscale wires is responsive to an electrical property external to the nanoscale wire.

10. The article of claim 1 , wherein the cell scaffold has a density of nanoscale wires of at least about 30 nanoscale wires/mm 3 .

11. The article of claim 1 , wherein at least about 50% of the nanoscale wires within the cell scaffold are individually electronically addressable, such that voltage or current may be applied to a nanoscale wire without simultaneously applying the voltage or the current to another nanoscale wire.

12. The article of claim 1 , wherein at least one of the polymeric constructs contains a metal lead extending between a nanoscale wire and a surface of the cell scaffold.

13. The article of claim 1 , wherein at least 50% of the nanoscale wires within the cell scaffold form portions of one or more electrical circuits extending externally of the cell scaffold.

14. The article of claim 1 , wherein the article further comprises cells on the cell scaffold.

15. The article of claim 1 , wherein the article further comprises an in vitro biological tissue containing the cell scaffold.

16. The article of claim 15 , wherein the biological tissue is configured to be implanted in an organism.

17. An article, comprising:
a 3-dimensional structure having an average pore size of between about 100 micrometers and about 1.5 mm, the structure comprising a curled and/or folded 2-dimensional electrical network comprising semiconductor nanoscale wires, wherein at least about 50% of the semiconductor nanoscale wires within the electrical network are individually electronically addressable, such that voltage or current may be applied to a nanoscale wire without simultaneously applying the voltage or the current to another nanoscale wire.

18. The article of claim 17 , wherein the curled 2-dimensional electrical network is curled into a cylinder having a maximum diameter of no more than about 5 mm.

Assignees

• Lieber Charles M
• Liu Jia
• Tian Bozhi
• Dvir Tal
• Langer Robert S
• Kohane Daniel S
• Harvard College

Inventors

• Lieber Charles M
• Liu Jia
• Tian Bozhi
• Dvir Tal
• Langer Robert S
• Kohane Daniel S

Classifications

• A61L2400/12

  Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces · CPC title

• A61L27/50

  Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} · CPC title

• A61L27/025

  Other specific inorganic materials not covered by A61L27/04 - A61L27/12 · CPC title

• C12M35/02

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M25/14

  Scaffolds; Matrices (in general C12N5/0068) · CPC title