Systems and methods for quantifying an analyte extracted from a sample

Patent metadata

Field	Value
Publication number	US-12374538-B2
Application number	US-202318526244-A
Country	US
Kind code	B2
Filing date	Dec 1, 2023
Priority date	Feb 21, 2014
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.

First claim

What is claimed is:

1. A method for analyzing a reaction, the method comprising:
introducing a solvent into a capillary;
introducing the capillary into a vessel comprising a sample comprising an analyte and a reactant such that a portion of the sample comprising the analyte and the reactant is introduced into the capillary;
allowing the analyte and the reactant to react with each other in the capillary to form a reaction product, which reaction continues over time along with formation of the reaction product over time;
moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent while the sample and the solvent remain separate from each other, thereby causing the reaction product to be extracted from the sample and into the solvent;
analyzing a first amount of formation of the reaction product that has been extracted from the sample at a first time point; and
repeating the moving and analyzing steps at least one more time to analyze at least a second amount of formation of the reaction product at least at a second time point, thereby monitoring reaction product formation over time.

2. The method according to claim 1 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and
analyzing the ions.

3. The method according to claim 1 , wherein analyzing comprises:
removing the solvent comprising the extracted reaction product from the capillary; and
conducting an assay that analyzes the reaction product.

4. The method according to claim 3 , wherein the assay comprises:
generating ions of the reaction product; and
analyzing the ions.

5. The method according to claim 1 , wherein the solvent comprises an internal standard.

6. The method according to claim 1 , wherein the sample comprises an internal standard.

7. The method according to claim 1 , wherein the solvent is immiscible with the sample.

8. The method according to claim 1 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

9. A method for analyzing an enzymatic reaction, the method comprising:
introducing a solvent into a capillary;
introducing the capillary into a vessel comprising a sample comprising an enzyme and a reactant such that a portion of the sample comprising the enzyme and the reactant is introduced into the capillary;
allowing the enzyme and the reactant to react with each other in the capillary to form a reaction product, which reaction continues over time along with formation of the reaction product over time;
moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent while the sample and the solvent remain separate from each other, thereby causing the reaction product to be extracted from the sample and into the solvent;
analyzing a first amount of formation of the reaction product that has been extracted from the sample at a first time point; and
repeating the moving and analyzing steps at least one more time to analyze at least a second amount of formation of the reaction product at least at a second time point, thereby monitoring reaction product formation over time.

10. The method according to claim 9 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and
analyzing the ions.

11. The method according to claim 9 , wherein analyzing comprises:
removing the solvent comprising the extracted reaction product from the capillary; and
conducting an assay that analyzes the reaction product.

12. The method according to claim 11 , wherein the assay comprises:
generating ions of the reaction product; and
analyzing the ions.

13. The method according to claim 9 , wherein the solvent is introduced to the capillary first.

14. The method according to claim 9 , wherein the sample is introduced to the capillary first.

15. The method according to claim 9 , wherein the solvent comprises an internal standard.

16. The method according to claim 9 , wherein the solvent is immiscible with the sample.

17. The method according to claim 9 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Ren Yue
• Lin Ziqing

Classifications

• Y10T436/24

  Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry · CPC title

• Y10T436/255

  including use of a solid sorbent, semipermeable membrane, or liquid extraction · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• H01J49/167 Primary

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title