Systems and methods for analyzing an extracted sample

Patent metadata

Field	Value
Publication number	US-10008375-B2
Application number	US-201414426591-A
Country	US
Kind code	B2
Filing date	Jan 10, 2014
Priority date	Jan 31, 2013
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

The invention generally relates to systems for analyzing a sample and methods of use thereof. In certain aspects, the invention provides systems that include an ionization probe and a mass analyzer. The probe includes a hollow body that has a distal tip. The probe also includes a substrate that is at least partially disposed within the body and positioned prior to the distal tip so that sample extracted from the substrate flows into the body prior to exiting the distal tip. The probe also includes an electrode that operably interacts with sample extracted from the substrate.

First claim

What is claimed is:

1. A system for analyzing a sample, the system comprising:
an ionization probe, the probe comprising:
a hollow body that comprises a distal tip;
a paper substrate configured to hold a sample, the paper substrate being at least partially disposed within the hollow body and positioned prior to the distal tip such that an analyte in the sample extracted from the paper substrate by a solvent flows into the hollow body prior to exiting the distal tip, wherein the hollow body is devoid of separation material after the paper substrate; and
an electrode disposed prior to the distal tip of the hollow body that operably interacts with the extracted analyte in the solvent to expel the sample from the distal tip and produce ions of the analyte; and

a mass analyzer operably coupled to the probe to receive the ions of the probe.

2. The system according to claim 1 , wherein the hollow body is composed of glass.

3. The system according to claim 1 , wherein the paper substrate is filter paper.

4. The system according to claim 1 , wherein the mass analyzer is for a mass spectrometer or a miniature mass spectrometer.

5. The system according to claim 4 , wherein the mass analyzer is selected from the group consisting of: a quadrupole ion trap, a rectalinear ion trap, a cylindrical ion trap, a ion cyclotron resonance trap, and an orbitrap.

6. The system according to claim 1 , further comprising a source of nebulizing gas.

7. The system according to claim 6 , wherein the source of nebulizing gas is configured to provide pulses of gas.

8. The system according to claim 6 , wherein the source of nebulizing gas is configured to provide a continuous flow of gas.

9. A method for analyzing a sample, the method comprising:
introducing a solvent to a sample held by a paper substrate that is at least partially disposed within a hollow body comprising a distal tip, wherein the solvents interacts with the sample held by the paper substrate to extract an analyte from the sample into the solvent, wherein the hollow body is devoid of separation material after the paper substrate;
applying a voltage to the extracted analyte in the solvent in the hollow body from an electrode disposed prior to the distal tip of the hollow body to expel the sample from the distal tip of the body, thereby generating ions of the analyte; and
analyzing the ions.

10. The method according to claim 9 , wherein a nebulizing gas is also applied to the extracted sample.

11. The method according to claim 10 , wherein the nebulizing gas is pulsed.

12. The method according to claim 10 , wherein the nebulizing gas is provided as a continuous flow of gas.

13. The method according to claim 9 , wherein the paper substrate is filter paper.

14. The method according to claim 9 , wherein the mass analyzer is for a mass spectrometer or a miniature mass spectrometer.

15. The method according to claim 9 , wherein the sample is introduced to the paper substrate prior to the paper substrate being inserted into the hollow body.

16. The method according to claim 9 , wherein the sample is introduced to the paper substrate after the paper substrate has been inserted into the hollow body.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Ren Yue
• Liu Jiangjiang
• Li Linfan

Classifications

• H01J49/167 Primary

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title

• H01J49/4205

  Device types · CPC title

• H01J49/0409 Primary

  Sample holders or containers (containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543) · CPC title

• H01J49/0431

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title

• H01J49/0459

  for solid samples · CPC title