Systems and methods for quantifying an analyte extracted from a sample

Patent metadata

Field	Value
Publication number	US-11495448-B2
Application number	US-202016985553-A
Country	US
Kind code	B2
Filing date	Aug 5, 2020
Priority date	Feb 21, 2014
Publication date	Nov 8, 2022
Grant date	Nov 8, 2022

Abstract

The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.

First claim

What is claimed is:

1. A method for extracting an analyte from a sample, the method comprising:
estimating by a partitioning coefficient a distribution of an analyte between a sample and a solvent to thereby determine a solubility of a target analyte in a solvent;
selecting the solvent that (i) will extract the target analyte from a sample into the solvent based on the target analyte being more soluble in the solvent than the sample and (ii) be compatible for ionization of the target analyte;
introducing the solvent into a capillary;
introducing the capillary into a vessel comprising the sample such that a portion of the sample is introduced into the capillary;
moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the target analyte to be extracted from the sample and into the solvent, wherein the sample and the solvent do not mix with each other;
analyzing the target analyte that has been extracted from the sample; and
quantifying the target analyte.

2. The method according to claim 1 , wherein the quantifying step is performed without knowledge of a volume of the sample.

3. The method according to claim 1 , wherein the quantifying step is performed without knowledge of a volume of the solvent.

4. The method according to claim 1 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and
analyzing the ions.

5. The method according to claim 1 , wherein analyzing comprises:
removing the solvent comprising the extracted analyte from the capillary; and
conducting an assay that analyzes the analyte.

6. The method according to claim 5 , wherein the assay comprises:
generating ions of the analyte; and
analyzing the ions.

7. The method according to claim 1 , wherein the solvent comprises an internal standard.

8. The method according to claim 1 , wherein the sample comprises an internal standard.

9. The method according to claim 1 , wherein the solvent is immiscible with the sample.

10. The method according to claim 1 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

11. A method for extracting an analyte from a sample, the method comprising:
estimating by a partitioning coefficient (LogP) a distribution of an analyte between a sample and a solvent to thereby determine a solubility of a target analyte in a solvent;
selecting the solvent that (i) will extract the target analyte from a sample into the solvent based on the target analyte being more soluble in the solvent than the sample and (ii) be compatible for ionization of the target analyte;
introducing the solvent into a capillary;
introducing the capillary into a vessel comprising the sample such that a portion of the sample is introduced into the capillary;
moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the target analyte to be extracted from the sample and into the solvent, wherein the sample and the solvent do not mix with each other.

12. The method according to claim 11 , further comprising analyzing the extracted analyte.

13. The method according to claim 12 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and
analyzing the ions.

14. The method according to claim 12 , wherein analyzing comprises:
removing the solvent comprising the extracted analyte from the capillary; and
conducting an assay that analyzes the analyte.

15. The method according to claim 14 , wherein the assay comprises:
generating ions of the analyte; and
analyzing the ions.

16. The method according to claim 11 , wherein the solvent is introduced to the capillary first.

17. The method according to claim 11 , wherein the sample is introduced to the capillary first.

18. The method according to claim 11 , wherein the solvent comprises an internal standard.

19. The method according to claim 11 , wherein the solvent is immiscible with the sample.

20. The method according to claim 11 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Ren Yue
• Lin Ziqing

Classifications

• Y10T436/24

  Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry · CPC title

• Y10T436/255

  including use of a solid sorbent, semipermeable membrane, or liquid extraction · CPC title

• H01J49/167 Primary

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title