Transaminase biocatalysts

Patent metadata

Field	Value
Publication number	US-12344874-B2
Application number	US-202117361832-A
Country	US
Kind code	B2
Filing date	Jun 29, 2021
Priority date	Feb 26, 2009
Publication date	Jul 1, 2025
Grant date	Jul 1, 2025

Abstract

The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

First claim

What is claimed is:

1. A recombinant transaminase polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 2, wherein the amino acid sequence of the recombinant transaminase polypeptide further comprises a residue difference as compared to SEQ ID NO: 2 at residue position X65 and at residue position X81.

2. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7 (8H)-yl]-1-(2,4,5-trifluorophenyl) butan-2-one (“ketoamide substrate”) to (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7 (8H)-yl]-1-(2,4,5-trifluorophenyl) butan-2-amine (“product”) under a defined reaction condition in the presence of an amino group donor isopropylamine to levels of the product detectable by HPLC-UV at 210 nm, where the reaction condition comprises about 2 g/L ketoamide substrate, about 0.5 M isopropylamine, about 22° C., about pH 7.5, about 5% DMSO, about 100 μM pyridoxal phosphate, and about 20 mg/mL of transaminase polypeptide.

3. The recombinant transaminase polypeptide of claim 2 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to the product with an activity that is equal to or greater than the activity of the polypeptide of SEQ ID NO: 4 under the defined reaction condition.

4. The recombinant transaminase polypeptide of claim 2 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 90% enantiomeric excess.

5. The recombinant transaminase polypeptide of claim 2 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 99% enantiomeric excess.

6. The transaminase polypeptide of claim 1 , wherein the amino acid sequence corresponds to the sequence of SEQ ID NO: 68, 70, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, or 168.

7. The transaminase polypeptide of claim 1 , wherein the amino acid sequence includes the following features: X69 is C or T; X122 is M or I; X223 is P; and X284 is G.

8. The transaminase polypeptide of claim 1 , wherein the amino acid sequence includes the following features: X69 is C or T; X122 is M or I; X223 is P; and X284 is G.

9. The transaminase polypeptide of claim 1 , wherein the amino acid sequence additionally comprises a residue difference as compared to SEQ ID NO:2 at one or more of the following residue positions: X4; X5; X8; X18; X25; X26; X27; X28; X30; X41; X42; X48; X49; X50; X54; X55; X60; X61; X62; X94; X96; X102; X117; X120; X124; X126; X136; X137; X138; X146; X148; X150; X152; X155; X156; X160; X163; X164; X169; X174; X178; X195; X199; X204; X208; X209; X211; X215; X217; X225; X230; X252; X269; X273; X282, X292; X297; X302; X306; X321; and X329.

Assignees

• Codexis Inc

Inventors

• Savile Christopher K
• Mundorff Emily
• Moore Jeffrey C
• Devine Paul N
• Janey Jacob M

Classifications

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title

• C12Y206/01018

  Beta-alanine-pyruvate transaminase (2.6.1.18) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N9/1096

  transferring nitrogenous groups (2.6) · CPC title

• C12P13/001

  Amines; Imines · CPC title