Transaminase biocatalysts

Patent metadata

Field	Value
Publication number	US-9550982-B2
Application number	US-201615140809-A
Country	US
Kind code	B2
Filing date	Apr 28, 2016
Priority date	Feb 26, 2009
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

First claim

What is claimed is:

1. A recombinant polynucleotide encoding a recombinant transaminase polypeptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2, wherein the amino acid residue at position 284 of said amino acid sequence is glycine and wherein the encoded recombinant transaminase polypeptide is capable of converting a ketoamide substrate to the product with an activity that is equal to or greater than the activity of the polypeptide of SEQ ID NO: 4 under a defined reaction condition.

2. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide comprises an aliphatic amino acid residue at position 122.

3. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide comprises a residue at position 122 selected from isoleucine, methionine, valine, and histidine.

4. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide is capable of converting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one (“ketoamide substrate”) to (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine (“product”) under a defined reaction condition in the presence of an amino group donor isopropylamine to levels of the product detectable by HPLC-UV at 210 nm, where the reaction condition comprises about 2 g/L ketoamide substrate, about 0.5 M isopropylamine, about 22° C., about pH 7.5, about 5% DMSO, about 100 μM pyridoxal phosphate, and about 20 mg/mL of transaminase polypeptide.

5. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 90% enantiomeric excess.

6. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 99% enantiomeric excess.

7. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:4.

8. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide, wherein the amino acid sequence of the encoded transaminase further comprises a residue difference as compared to SEQ ID NO:2 at one or more residue positions selected from: X62, X69, X136, X137, X195, X199, X208, X209, X225, and X282.

9. The transaminase polynucleotide of claim 8 , wherein the residue difference encoded recombinant transaminase polypeptide occurs at one or more residue positions selected from: X62, X69, X136, X137, X195, X199, X208, X209, X225, and X282.

10. The transaminase polynucleotide of claim 8 , wherein the encoded recombinant transaminase polypeptide comprising a cysteine, or a non-polar, polar, or aliphatic amino acid at position X69.

11. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide further comprises at least one residue difference as compared to SEQ ID NO:2, at one or more of the following residue positions: X4; X5; X8; X18; X25; X26; X27; X28; X30; X41; X42; X48; X49; X50; X54; X55; X60; X61; X62; X65; X81; X94; X96; X102; X117; X120; X124; X126; X136; X137; X138; X146; X148; X150; X152; X155; X156; X160; X163; X164; X169; X174; X178; X195; X199; X204; X208; X209; X211; X215; X217; X225; X230; X252; X269; X273; X282, X292; X297; X302; X306; X321; and X329.

12. The transaminase polynucleotide of claim 11 , wherein the encoded recombinant transaminase comprises the amino acid residue differences selected from the following:
X4 is an aromatic residue;
X5 is a basic residue;
X8 is a constrained residue;
X18 is a cysteine or an aliphatic residue;
X25 is a polar residue;
X26 is an aromatic or constrained residue;
X27 is a polar residue;
X28 is a constrained residue;
X30 is polar or non-polar residue;
X41 is a constrained or polar residue;
X42 is non-polar residue;
X48 is a polar, acidic, aliphatic or non-polar residue;
X49 is a polar residue;
X50 is an aliphatic residue;
X54 is a constrained residue;
X55 is an aliphatic residue;
X60 is an aromatic residue;
X61 is an aromatic residue;
X62 is an aromatic or polar residue;
X65 is an aliphatic residue;
X81 is a non-polar or small residue;
X94 is an aliphatic residue;
X96 is an aliphatic residue;
X102 is an aliphatic or basic residue;
X117 is a non-polar residue;
X120 is an aromatic residue;
X124 is a polar or constrained residue;
X126 is a polar residue;
X136 is an aromatic residue;
X137 is a polar or aliphatic residue;
X138 is a basic or constrained residue;
X146 is a basic residue;
X148 is an aliphatic or aromatic residue;
X150 is aromatic, constrained or polar residue;
X152 is C, or a non-polar, aliphatic, or polar residue;
X155 is non-polar or polar residue;
X156 is a polar residue;
X160 is an aliphatic residue;
X163 is an aliphatic or constrained residue;
X164 is an aliphatic or constrained residue;
X169 is an aliphatic residue;
X174 is an aliphatic residue;
X178 is a polar residue;
X195 is an aromatic or polar residue;
X199 is an aliphatic or aromatic residue;
X204 is an aliphatic residue;
X208 is cysteine, or a constrained, non-polar, aromatic, polar, or basic residue;
X209 is an aliphatic residue;
X211 is an aliphatic residue;
X215 is cysteine;
X217 is a polar residue;
X225 is an aromatic residue;
X230 is an aliphatic residue;
X252 is an aromatic residue;
X269 is a constrained residue;
X273 is an aromatic residue;
X282 is a polar residue;
X292 is a polar residue;
X297 is a polar residue;
X302 is an aliphatic residue;
X306 is an aliphatic residue;
X321 is a constrained residue; and
X329 is a constrained or aromatic residue.

13. The transaminase polynucleotide of claim 11 , wherein the encoded recombinant transaminase polypeptide, wherein the amino acid residue differences are selected from the following:
X4 is Y;
X5 is K;
X8 is P;
X18 is C or I;
X25 is Q;
X26 is H;
X27 is T;
X28 is P;
X30 is Q or M;
X41 is H or S;
X42 is G;
X48 is Q, D, V, G, or A;
X49 is T;
X50 is L;
X54 is P or H;
X55 is V;
X60 is F;
X61 is Y;
X62 is T, Y or F;
X65 is A;
X81 is G;
X94 is I or L;
X96 is L;
X102 is L or K;
X117 is G;
X120 is Y;
X124 is T, H or N;
X126 is T;
X136 is Y or F;
X137 is T or I;
X138 is K or P;
X146 is R;
X148 is A or F;
X150 is F, H, or S;
X152 is G, I, L, S or C;
X155 is M, V or T;
X156 is Q;
X160 is L;
X163 is H or V;
X164 is V or P;
X169 is L;
X174 is A;
X178 is S;
X195 is F or Q;
X199 is W or I;
X204 is A;
X208 is H, C, G, K, N, Y, D or S;
X209 is L;
X211 is I;
X215 is C;
X217 is N;
X225 is Y;
X230 is V;
X252 is F;
X269 is P;
X273 is Y;
X282 is S;
X292 is T;
X297 is S;
X302 is A;
X306 is L;
X321 is P; and
X329 is H.

14. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product with at least 50 to 100 times or greater activity than the polypeptide of SEQ ID NO:4.

15. The transaminase polynucleotide of claim 1 , wherein the encoded recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product with at least 1.1 to 5 times or greater activity than the polypeptide of SEQ ID NO:22, 48, or 58.

16.

Assignees

• Codexis Inc

Inventors

• Savile Christopher K
• Mundorff Emily
• Moore Jeffrey C
• Devine Paul N
• Janey Jacob M

Classifications

• C12P13/001

  Amines; Imines · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title

• C12Y206/01018

  Beta-alanine-pyruvate transaminase (2.6.1.18) · CPC title

• C12N9/1096 Primary

  transferring nitrogenous groups (2.6) · CPC title