Compositions and methods for producing benzylisoquinoline alkaloids
US-2016304923-A1 · Oct 20, 2016 · US
Transaminase biocatalysts
US9944963B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9944963-B2 |
| Application number | US-201615376837-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 13, 2016 |
| Priority date | Feb 26, 2009 |
| Publication date | Apr 17, 2018 |
| Grant date | Apr 17, 2018 |
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- Title
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Abstract
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The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
First claim
Opening claim text (preview).
What is claimed is: 1. A recombinant transaminase polypeptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:110, wherein said recombinant transaminase polypeptide is capable of converting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one (“ketoamide substrate”) to (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine (“product”) under a defined reaction condition in the presence of an amino group donor isopropylamine to levels of the product detectable by HPLC-UV at 210 nm, where the reaction condition comprises about 2 g/L ketoamide substrate, about 0.5 M isopropylamine, about 22° C., about pH 7.5, about 5% DMSO, about 100 μM pyridoxal phosphate, and about 20 mg/mL of transaminase polypeptide. 2. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to the product with an activity that is equal to or greater than the activity of the polypeptide of SEQ ID NO: 4 under the defined reaction condition. 3. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 90% enantiomeric excess. 4. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 99% enantiomeric excess. 5. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product with at least 50 to 100 times or greater activity than the polypeptide of SEQ ID NO:4. 6. The recombinant transaminase polypeptide of claim 1 , wherein said encoded recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product with at least 1.1 to 5 times or greater activity than the polypeptide of SEQ ID NO:22, 48, or 58.
Assignees
Inventors
Classifications
Beta-alanine-pyruvate transaminase (2.6.1.18) · CPC title
- C12P17/182Primary
Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system (alloxazine or isoalloxazine, e.g. riboflavine C12P25/00) · CPC title
transferring nitrogenous groups (2.6) · CPC title
Amines; Imines · CPC title
using catalysts, e.g. selective catalysts · CPC title
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Frequently asked questions
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- What does patent US9944963B2 cover?
- The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P17/182. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 17 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).