Manufacturing method of the operation pipe

Patent metadata

Field	Value
Publication number	US-12263489-B2
Application number	US-202217840568-A
Country	US
Kind code	B2
Filing date	Jun 14, 2022
Priority date	Mar 9, 2019
Publication date	Apr 1, 2025
Grant date	Apr 1, 2025

Abstract

A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

First claim

What is claimed is:

1. A manufacturing method of an operation pipe for operating target components, comprising:
providing a hollow pipe having a closable open end for supplying a sample containing the target components on one side and a closed end on the other side, and having an operation pipe portion on the open end side and a recovery pipe portion on the closed end side;
filling a recovery medium in the recovery pipe portion so that a first gel layer and a first aqueous liquid layer which is in contact with the closed end are multi-layered, wherein the first aqueous liquid layer in contact with the closed end has a predetermined volume, a layer length of the first aqueous liquid layer in contact with the closed end varies with variations in an inner diameter of the hollow pipe or a length of the hollow pipe, and a volume of the first aqueous liquid layer in contact with the closed end is 50 μL-300 μL, and a layer length of the first gel layer is determined by a length in the longitudinal direction of the hollow pipe;
filling an operation medium in the operation pipe portion so that second gel layers and second aqueous liquid layers are alternately multi-layered in the longitudinal direction of the hollow pipe, wherein a layer length of each of the second gel layers and a layer length of each of the second aqueous liquid layers are determined by the length in the longitudinal direction of the hollow pipe.

2. The manufacturing method of the operation pipe according to claim 1 , wherein the inner diameter of the hollow pipe is 0.1 mm-5 mm.

3. The manufacturing method of the operation pipe according to claim 1 , wherein the operation pipe portion and the recovery pipe portion are separable.

4. The manufacturing method of the operation pipe according to claim 1 , wherein the material of the hollow pipe is selected from a group consisting of polyethylene, polypropylene, fluororesin, polyvinyl chloride, polystyrene, polycarbonate, acrylonitrile- butadiene-styrene copolymer, acrylonitrile-styrene copolymer, acrylic resin, polyvinyl acetate, polyethylene terephthalate, cyclic polyolefin, and glass.

5. The manufacturing method of the operation pipe according to claim 1 , wherein an inner diameter of the open end is larger than an inner diameter of the operation pipe portion and an inner diameter of the recovery pipe portion.

6. The manufacturing method of the operation pipe according to claim 1 , wherein the hollow pipe has optical transparency.

7. The manufacturing method of the operation pipe according to claim 1 , wherein surface roughness of an inner surface of the hollow pipe is 0.1 μm or less.

8. The manufacturing method of the operation pipe according to claim 1 , wherein the layer length of the first gel layer and the layer length of each of the second gel layers are 1-20 mm.

9. The manufacturing method of the operation pipe according to claim 1 , wherein the layer length of each of the second aqueous liquid layers is 0.5-30 mm.

Assignees

• Shimadzu Corp

Inventors

• Shikata Masamitsu
• Minamimoto Ayaka
• Muramatsu Akira
• Kanai Masaki
• Fujiwara Seiya
• Ohashi Tetsuo
• Jikuya Hiroyuki

Classifications

• B01L2400/043

  magnetic forces · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12N15/1013

  by using magnetic beads · CPC title

• B01L9/06

  Test-tube stands; Test-tube holders · CPC title